A simple method for the DNA content assay of culture maintained pancreatic islets of Langerhans

A simple method for assaying DNA content of in vitro culture maintained pancreatic islets is described. By employing lyophilization of the islets and subsequent fluorometric analysis, we have been able to measure DNA from relatively small cell samples. The method may result advantageous, over others...

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Bibliographic Details
Published inJournal of endocrinological investigation Vol. 14; no. 8; p. 629
Main Authors Calafiore, R, Falorni, Jr, A, Ambrosini, M V, Basta, G, Picchio, M L, Brunetti, P
Format Journal Article
LanguageEnglish
Published Italy 01.09.1991
Subjects
Ammonium Hydroxide
Animals
Cells, Cultured
Detergents
DNA - analysis
Dogs
Female
Freeze Drying
Hydroxides
Islets of Langerhans - chemistry
Lymphocytes - chemistry
Male
Octoxynol
Polyethylene Glycols
Rats
Rats, Inbred Lew
Reference Standards
Spectrometry, Fluorescence
Swine
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Summary:A simple method for assaying DNA content of in vitro culture maintained pancreatic islets is described. By employing lyophilization of the islets and subsequent fluorometric analysis, we have been able to measure DNA from relatively small cell samples. The method may result advantageous, over others, previously reported, since it is less subject to experiment-related interfering conditions. This procedure, which is easy and reliable, even for very low DNA concentrations, seems to be very useful for culture maintained pancreatic islets, since in vitro work conducted with this endocrine tissue is usually associated with small size cell samples. The method may help for a simple assessment of the viable islet cell mass in in vitro studies.
ISSN:0391-4097
1720-8386
DOI:10.1007/BF03347882