HPLC Determination of Nitrated Polycyclic Aromatic Hydrocarbons after Their Reduction to Amino Derivatives

• Published in: Analytical letters Vol. 37; no. 11; pp. 2379 - 2392
• Main Authors: Brichac, Jiri, Zima, Jiri, Barek, Jiri
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA Taylor & Francis Group 27.12.2004; Taylor & Francis
• Subjects: Analytical chemistry; Chemistry; Chromatographic methods and physical methods associated with chromatography; Exact sciences and technology; Fluorescence detection; HPLC; Nitrated polycyclic aromatic hydrocarbons (NPAH); Other chromatographic methods; Reduction by titanium(III); Water; Fluorescence detector; Hydrocarbon; Titanium complex; HPLC chromatography; Citrate; Nitrated polycyclic aromatic hydrocarbons (NPAH); Polycyclic aromatic compound; Reducing agent; Titanium III; Fluorescence detection; Hydrochloric acid; Detection limit; Reduction by titanium(III); Acid medium; HPLC
• ISSN: 0003-2719; 1532-236X
• DOI: 10.1081/AL-200028178

High-performance liquid chromatography with fluorescence detection (HPLC-FD) was used for the determination of 1-nitropyrene, 2-nitrofluorene, and 6-nitrochrysene after their reduction to amino derivatives. As a reducing agent, titanium(III) complexed with citrate in a hydrochloric acid medium was used. Conditions for 1-nitropyrene, 2-nitrofluorene, and 6-nitrochrysene reduction, and for separation of corresponding amino derivatives were found. The limit of detection was 68 pg for 2-nitrofluorene, 82 pg for 2-nitropyrene, and 629 pg for 6-nitrochrysene. The applicability of this method for the determination of 1-nitropyrene in water samples was verified.