Biocompatible calcium phosphate based tubes

• Published in: Journal of materials chemistry Vol. 2; no. 33; pp. 6923 - 6928
• Main Authors: Chandanshive, Balasaheb, Dyondi, Deepti, Ajgaonkar, Vishnu R, Banerjee, Rinti, Khushalani, Deepa
• Format: Journal Article
• Language: English
• Published: 07.09.2010
• Subjects: Aluminum oxide; Biocompatibility; Bones; Calcium phosphate; Cellular; Etching; Fibroblasts; Nanotubes; Tubes
• ISSN: 0959-9428; 1364-5501
• DOI: 10.1039/c0jm00145g

Porous anodic alumina template has been employed, in the presence of a precipitation reaction involving Ca(OH) 2 and H 3 PO 4 , to form calcium phosphate based tubular structures. These structures are amorphous in nature and can be recovered by etching the sacrificial alumina membrane. Full characterization of these structures has been done using X-ray diffraction, electron microscopy and FTIR. In addition, their biocompatibility has been tested on L929 mouse fibroblast cells using MTT assay and the cellular internalization of these nanotubes has also been evaluated using rhodamine 6G dye tagged nanotubes in the presence of fibroblast cells. The studies also suggest that the nanotubes are non-toxic to fibroblasts and can be taken up easily by mammalian cells. Such tubes may serve as vehicles for drugs and growth factors, and for tissue repair including bone regeneration. Amorphous calcium phosphate tubular structures have been formed and their biocompatibility has been tested on L929 mouse fibroblast cells using an MTT assay and the successful cellular uptake of these nanotubes was evaluated using rhodamine B dye.