Characterization of bolting behaviour in garlic (Allium sativum) using SNP
The present research was conducted to evaluate the bolting (incomplete bolters and non-bolters) behaviour in garlic (Allium sativum L.) accessions collected from different geographical areas. A total of 96 genotypes collected from different sources were used for genotype sequencing to identify SNP b...
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Published in | The Indian journal of agricultural sciences Vol. 90; no. 1; pp. 112 - 117 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Indian Council of Agricultural Research
01.01.2020
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Subjects | |
Online Access | Get full text |
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Summary: | The present research was conducted to evaluate the bolting (incomplete bolters and non-bolters) behaviour in garlic (Allium sativum L.) accessions collected from different geographical areas. A total of 96 genotypes collected from different sources were used for genotype sequencing to identify SNP but only 73 genotypes among them showed good amplification. A primer (AsLFY) was designed by using reference of already designed gene specific primer (gALFY-2) by Rotem et al. (2007). After amplification, sequencing of these 73 genotypes was done; SNP (CCGTCG) was at 301 bp from initiation codon, i.e. G for non-bolters genotypes and A for incomplete bolter genotypes. Thus, the identified SNP would help in detecting incomplete bolter and non-bolter genotypes at seedling stage which can be useful for garlic breeding programmes. |
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ISSN: | 0019-5022 2394-3319 |
DOI: | 10.56093/ijas.v90i1.98556 |