Identification of leukemia cell-derived inhibitory activity (LIA) in conditioned media from human myeloid leukemic cell line ML-2

Conditioned media from the human myeloid leukemic cell line ML-2 contain a factor that inhibits the entry of normal CFU-GM into S phase of mitotic cycle as measured by the 3H-TdR suicide technique. This factor was detected in conditioned media prepared by incubating 5 X 10(6) ML-2 cells/ml or 1 X 10...

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Bibliographic Details
Published inBlut Vol. 52; no. 1; p. 51
Main Authors Cukrová, V, Hrkal, Z, Koprivová, H, Neuwirt, J
Format Journal Article
LanguageEnglish
Published Germany 01.01.1986
Subjects
Animals
Bone Marrow
Cell Line
Chromatography, Gel
Colony-Forming Units Assay
Colony-Stimulating Factors - analysis
Colony-Stimulating Factors - antagonists & inhibitors
Culture Media - analysis
Ferritins - pharmacology
Granulocytes
Hematopoiesis
Hematopoietic Stem Cells
Humans
Isoelectric Focusing
Leukemia, Myeloid
Macrophages
Male
Mice
Placenta
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Summary:Conditioned media from the human myeloid leukemic cell line ML-2 contain a factor that inhibits the entry of normal CFU-GM into S phase of mitotic cycle as measured by the 3H-TdR suicide technique. This factor was detected in conditioned media prepared by incubating 5 X 10(6) ML-2 cells/ml or 1 X 10(6) ML-2 cells/ml in serum-free RPMI for 5 or 24 hours respectively, and was isolated by ultrafiltration through an XM 300 Diaflo membrane followed by chromatography on Sepharose 6 B. Ferritin, prepared from human placenta, had the same inhibitory effect on CFU-GM. Antibodies against human placental ferritin completely inactivated the inhibitory effect of both human placental ferritin and the factor released from ML-2 cells. The inhibitory activity produced by the cell-line ML-2 was considered as LIA (leukemia cell-derived inhibitory activity) earlier found in HL-60 cell line and AML and CML cells.
ISSN:0006-5242
DOI:10.1007/BF00320142