Construction of a dual-component hydrogel matrix for 3D biomimetic skin based on photo-crosslinked chondroitin sulfate/collagen

The main challenge in the field of 3D biomimetic skin is to search for a suitable hydrogel matrix with good biocompatibility, appropriate mechanical property and inner porosity that can support the adhesion and proliferation of skin cells. In this study, photocurable chondroitin sulfate methacrylate...

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Published inInternational journal of biological macromolecules Vol. 254; no. Pt 3; p. 127940
Main Authors He, Shengsheng, Li, Huijuan, Chi, Baiyi, Zhang, Xingjiang, Wang, Yuzhe, Wu, Jianxin, Huang, Qing
Format Journal Article
LanguageEnglish
Published Netherlands 01.01.2024
Subjects
Biomimetics
Chondroitin Sulfates - chemistry
Collagen - chemistry
Hydrogels - chemistry
Methacrylates - chemistry
Tissue Engineering - methods
Photo-crosslinked
Chondroitin sulfate
Hydrogel matrix
Biomimetic skin
Collagen
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Summary:The main challenge in the field of 3D biomimetic skin is to search for a suitable hydrogel matrix with good biocompatibility, appropriate mechanical property and inner porosity that can support the adhesion and proliferation of skin cells. In this study, photocurable chondroitin sulfate methacrylate (CSMA) and collagen methacrylate (CoLMA) synthesized from chondroitin sulfate (CS) and type I collagen I (CoL) in the dermal matrix were used to construct a photo-crosslinked dual-component CSMA-CoLMA hydrogel matrix. Due to the toughening effect of the dual-component, the CSMA-CoLMA hydrogel improved the intrinsic brittleness of the single-component CSMA hydrogel, presented good mechanical tunability. The average storage and elasticity modulus could reach 3.3 KPa and 30.3 KPa, respectively, which were close to those of natural skin. The CSMA-CoLMA hydrogel with a ratio of 8/6 showed suitable porous structure and good biocompatibility, supporting the adhesion and proliferation of skin cells. Furthermore, the expression of characteristic marker proteins was detected in the epidermal and dermal bi-layered models constructed with the hydrogel containing keratinocytes and fibroblasts. These results suggest that the dual-component CSMA-CoLMA hydrogel has promising potential as a matrix to construct 3D biomimetic skin.
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ISSN:0141-8130
1879-0003
DOI:10.1016/j.ijbiomac.2023.127940