Detection of calcitonin gene expression in human infant and monkey carotid body chief cells by in situ hybridization

Calcitonin mRNA was detected in human and monkey carotid bodies by in situ hybridization histochemistry, using a 35S-labeled oligonucleotide probe for human calcitonin. In both human and monkey carotid body, moderate to high hybridization signal for calcitonin mRNA was observed in all cases. The hyb...

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Bibliographic Details
Published inCell and tissue research Vol. 276; no. 2; p. 399
Main Authors Wang, Y Y, Cutz, E, Perrin, D G
Format Journal Article
LanguageEnglish
Published Germany 01.05.1994
Subjects
Animals
Base Sequence
Calcitonin - biosynthesis
Calcitonin - genetics
Carotid Body - cytology
Carotid Body - metabolism
Child, Preschool
Gene Expression
Humans
In Situ Hybridization
Infant
Infant, Newborn
Macaca fascicularis - genetics
Macaca fascicularis - metabolism
Molecular Sequence Data
Species Specificity
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Summary:Calcitonin mRNA was detected in human and monkey carotid bodies by in situ hybridization histochemistry, using a 35S-labeled oligonucleotide probe for human calcitonin. In both human and monkey carotid body, moderate to high hybridization signal for calcitonin mRNA was observed in all cases. The hybridization signal in the formalin-fixed, paraffin-embedded samples was comparable to that obtained from frozen paraformaldehyde-fixed tissue. Our observations extend the finding of calcitonin-like immunoreactivity in the carotid body chief cells and indicate that calcitonin is produced in the carotid body, probably in the chief cells.
ISSN:0302-766X
DOI:10.1007/BF00306125