Evaluation of the effect of Exercise Trainings and CGRP receptor antagonist (BIBN 4096) on mitochondrial dynamic in the hippocampus of male Wistar rats

• Published in: Neuroscience letters Vol. 828; p. 137752
• Main Authors: Shahouzehi, Beydolah, Masoumi-Ardakani, Yaser, Fallah, Hossein, Aminizadeh, Soheil
• Format: Journal Article
• Language: English
• Published: Ireland Elsevier B.V 01.04.2024
• Subjects: Bcl-2; CGRP; Endurance training; High-intensity interval training; Bcl-2; NO; NOS; Nrf-1; Sirt3; High-intensity interval training; Endurance training; Pgc-1 α; CGRP; HIIT; ET
• ISSN: 0304-3940; 1872-7972
• DOI: 10.1016/j.neulet.2024.137752

•CGRP receptor antagonist has no detrimental effects on rats’ hippocampus gene expression that are related to dynamic mitochondria.•Using of CGRP receptor antagonist can be helpful identifying and ultimately validating the beneficial effects of CGRPCGRPi.•Exercise training showed independent beneficial effects but the combination of exercise training and CGRP receptor antagonist have no additive effects. Exercise training showed beneficial effects on brain. The purpose of the present study is to evaluate the effect of six weeks of high-intensity interval training (HIIT) and Endurance training (ET) with calcitonin gene-related peptide (CGRP) receptor antagonist on the expression of genes involved in mitochondrial dynamics and apoptosis in hippocampal tissue of male Wistar rats. In this study, forty-two healthymale Wistar rats (8-week) were randomly divided into 6 groups (n = 7) as follow; 1) Control; 2) HIIT which performed 6 weeks of HIIT; 3) ET which performed 6 weeks of endurance training; 4) CGRPi received 10 mg/kg CGRP receptor antagonist every day at the last 2 weeks; 5) CGRPi-HIIT performed HIIT and received CGRP receptor antagonist; 6) CGRPi-ET performed ET and received CGRP receptor antagonist. Real-time PCR (2-ΔΔCT) and western blotting were employedto measure the expression of genes and protein, respectively. HIIT and ET significantly increased Bcl-2, Pgc-1α, Sirt3, and Nrf-1 gene expression in the hippocampal tissue (p < 0.05, p < 0.01, p < 0.01, and p < 0.001, respectively). ET-CGRPi and HIIT-CGRPi significantly increased Sirt3, Pgc-1α, and Nrf-1 gene expression compared to the control group (p < 0.05, p < 0.01, and p < 0.05, respectively). ET and HIIT-induced physiological alterations in the hippocampus. In fact, this modulation showed protective properties in the hippocampusvia up regulation of Bcl-2, Pgc-1α, Nrf-1, and Sirt3 gene expression. CGRPi did not cause gene or protein changes harmful to mitochondrial dynamic balance and apoptosis in the hippocampus of rats.