Deciphering Phosphotyrosine-Dependent Signaling Networks in Cancer by SH2 Profiling

It has been a decade since the introduction of SH2 profiling, a modular domain-based molecular diagnostics tool. This review covers the original concept of SH2 profiling, different analytical platforms, and their applications, from the detailed analysis of single proteins to broad screening in trans...

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Bibliographic Details
Published inGenes & cancer Vol. 3; no. 5-6; pp. 353 - 361
Main Authors Machida, Kazuya, Khenkhar, Malik, Nollau, Peter
Format Journal Article
LanguageEnglish
Published United States SAGE Publications 01.05.2012
Subjects
phosphoproteomics
cancer
SH2 domain
tyrosine phosphorylation
SH2 profiling
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Summary:It has been a decade since the introduction of SH2 profiling, a modular domain-based molecular diagnostics tool. This review covers the original concept of SH2 profiling, different analytical platforms, and their applications, from the detailed analysis of single proteins to broad screening in translational research. Illustrated by practical examples, we discuss the uniqueness and advantages of the approach as well as its limitations and challenges. We provide guidance for basic researchers and oncologists who may consider SH2 profiling in their respective cancer research, especially for those focusing on tyrosine phosphoproteomics. SH2 profiling can serve as an alternative phosphoproteomics tool to dissect aberrant tyrosine kinase pathways responsible for individual malignancies, with the goal of facilitating personalized diagnostics for the treatment of cancer.
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ISSN:1947-6019
1947-6027
DOI:10.1177/1947601912459048