Species and Organ differences of Sulphate Conjugation of p-Nitrophenol in Liver and Platelets

• Published in: Chemical & pharmaceutical bulletin Vol. 40; no. 7; pp. 1964 - 1965
• Main Authors: NAKAMURA, Junko, MIZUMA, Takashi, HAYASHI, Masahiro, AWAZU, Shoji
• Format: Journal Article
• Language: English
• Published: Tokyo The Pharmaceutical Society of Japan 25.07.1992; Maruzen
• Subjects: Analytical, structural and metabolic biochemistry; Animals; Biological and medical sciences; Blood Platelets - metabolism; Dogs; Enzymes and enzyme inhibitors; Fundamental and applied biological sciences. Psychology; Guinea Pigs; liver; Liver - metabolism; Male; Nitrophenols - blood; Nitrophenols - metabolism; Organ Specificity; p-nitrophenol; phenol sulphotransferase; platelet; Rabbits; Rats; species difference; Species Specificity; Sulfates - blood; Sulfates - metabolism; Transferases; Fissipedia; Carnivora; Enzyme; Interspecific comparison; Liver; Rodentia; Rabbit; Arylsulfotransferase; Lagomorpha; Cytosol; Vertebrata; Phenol; Platelet; Mammalia; Guinea pig; Animal; Dog; Sulfoconjugation; Organ
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.40.1964

Sulphate conjugation of p-nitrophenol (p-NP) in the liver and platelet cytosol of guinea pigs, rabbits and dogs were studied. The dependency of phenol sulphotransferase (PST) activity on p-NP concentration in the liver of guinea pigs and rabbits and in the platelets of guinea pigs were similar to that reported for the liver (Mizuma et al., J. Pharmacobio-Dyn., 6, 851 (1983)) and platelets (Nakamura et al., J. Pharm. Pharmacol., 42, 207 (1990)) of rats. There was one peak of PST activity on p-NP at the concentration of 1 to 10 μM, and the PST activity was increased again with an increase of p-NP concentration above the original concentration. On the other hand, a peak in PST activity on p-NP at the concentration of 1 to 10 μM was not observed in the platelets of rabbits and dogs. These results indicated species and organ differences in PST activity on p-NP in liver and platelets. The biphasic activities of the PST on p-NP in platelets and liver of rat and guinea pig were similar to that reported in humans (Reiter et al., Naunyn-Schmiedeberg's Arch. Pharmacol., 324, 140 (1983)).