Amino acid reference intervals by high performance liquid chromatography in plasma sample of Brazilian children

• Published in: Jornal brasileiro de patologia e medicina laboratorial Vol. 52; no. 2; pp. 70 - 77
• Main Authors: Cruz, Ana F., Barbosa, Tatiana Maria C. C., Adelino, Talita Émile R., Lima, William P., Mendes, Magda O., Valadares, Eugênia R.
• Format: Journal Article
• Language: English; Portuguese
• Published: Sociedade Brasileira de Patologia Clínica; Sociedade Brasileira de Patologia; Sociedade Brasileira de Citopatologia 01.04.2016; Sociedade Brasileira de Patologia Clínica
• Subjects: aminoácidos; cromatografia líquida; erros inatos do metabolismo; MEDICAL LABORATORY TECHNOLOGY; MEDICINE, RESEARCH & EXPERIMENTAL; PATHOLOGY; valores de referência; inborn errors of metabolism; reference values; valores de referência; aminoácidos; amino acids; liquid chromatography; cromatografia líquida; erros inatos do metabolismo
• ISSN: 1676-2444; 1678-4774; 1678-4774
• DOI: 10.5935/1676-2444.20160012

ABSTRACT Introduction: The high performance liquid chromatography is a technique used for quantification of amino acids in plasma. The definition of reference intervals in the population is very important for the diagnosis and monitoring of individuals with amino acid disorders. Objectives: The objectives of this study were to validate a method for amino acids quantification and define reference intervals in Brazilian children. Results: Good chromatographic separation was achieved using C18 solid-core column. The method showed satisfactory linearity, limits of detection and quantification, precision and accuracy. The reference ranges for aspartate, glutamate, asparagine, histidine, serine, glutamine, arginine, tyrosine, alanine, tryptophan, methionine, valine, phenylalanine, isoleucine and leucine were defined in a group of 60 healthy individuals aged 2 to 14 years. Conclusion: The presented technique can be applied in clinical practice. Each laboratory should preferably establish its own reference intervals. If that is not possible, it is recommended the use of the reference intervals described in this study for the diagnosis and monitoring of Brazilian children in this age group.