Production of aminoterminal peptide of type III Procollagen by established human Hepatoma cell lines

One cell line from human hepatoma, PLC/PRF/5, was found to produce type III procollagen, while four other ones, huH-1, huH-4, Huh-6c15 and Huh-7, were not. For the assay of aminoterminal peptide of type III procollagen, a radioimmunoassay was used. The productive rate of this peptide by PLC/PRF/5 wa...

Full description

Saved in:
Bibliographic Details
Published inKanzo Vol. 27; no. 9; pp. 1290 - 1295
Main Authors HATAHARA, Terumasa, IGARASHI, Shogo, FUNAKI, Naoya, NAKABAYASHI, Hidekazu, SATO, Jiro
Format Journal Article
LanguageJapanese
Published The Japan Society of Hepatology 1986
Subjects
Aminoterminal peptide of Type III Procollagen
Online AccessGet full text

Cover

More Information
Summary:One cell line from human hepatoma, PLC/PRF/5, was found to produce type III procollagen, while four other ones, huH-1, huH-4, Huh-6c15 and Huh-7, were not. For the assay of aminoterminal peptide of type III procollagen, a radioimmunoassay was used. The productive rate of this peptide by PLC/PRF/5 was one-fortieth of that of human embryonal fibroblasts. The spent media of the above hepatoma cell lines showed stimulative activity on human embryonal fibroblast for collagen synthesis, and the activity remained in the nondialyzable fraction. The surrounding tissue composed of fibroblasts might be mainly responcible for the increase of this peptide in the sera of patients with liver cancer.
ISSN:0451-4203
1881-3593
DOI:10.2957/kanzo.27.1290