Acetyl-CoA carboxylase 1 depletion suppresses de novo fatty acid synthesis and mitochondrial β-oxidation in castration-resistant prostate cancer cells

• Published in: The Journal of biological chemistry Vol. 299; no. 1; p. 102720
• Main Authors: Liu, Shaoyou, Lai, Jiarun, Feng, Yuanfa, Zhuo, Yangjia, Zhang, Hui, Chen, Yupeng, Li, Jinchuang, Mei, Xinyue, Zeng, Yanting, Su, Jiaming, Deng, Yulin, Jiang, Funeng, Yang, Shengbang, Tan, Huijing, Hon, Chi Tin, Wei, Sun, Han, Zhaodong, Wang, Fen, Zhong, Weide
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2023
• Subjects: Acetyl-CoA Carboxylase - metabolism; acetyl-CoA carboxylase 1; apoptosis; Cell Line, Tumor; de novo fatty acid synthesis; energy stress; Fatty Acids - metabolism; Humans; Male; Mitochondria - genetics; Mitochondria - metabolism; Phosphatidylinositol 3-Kinases - genetics; prognosis; prostate cancer; Prostatic Neoplasms, Castration-Resistant - genetics; Prostatic Neoplasms, Castration-Resistant - metabolism; de novo fatty acid synthesis; energy stress; apoptosis; GSEA; FACS; prognosis; TCGA; PCa; DFS; GEO; mCRPC; prostate cancer; acetyl-CoA carboxylase 1; FBS; ROS; cDNA
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1016/j.jbc.2022.102720

Cancer cells, including those of prostate cancer (PCa), often hijack intrinsic cell signaling to reprogram their metabolism. Part of this reprogramming includes the activation of de novo synthesis of fatty acids that not only serve as building blocks for membrane synthesis but also as energy sources for cell proliferation. However, how de novo fatty acid synthesis contributes to PCa progression is still poorly understood. Herein, by mining public datasets, we discovered that the expression of acetyl-CoA carboxylase alpha (ACACA), which encodes acetyl-CoA carboxylase 1 (ACC1), was highly expressed in human PCa. In addition, patients with high ACACA expression had a short disease-free survival time. We also reported that depletion of ACACA reduced de novo fatty acid synthesis and PI3K/AKT signaling in the human castration-resistant PCa (CRPC) cell lines DU145 and PC3. Furthermore, depletion of ACACA downregulates mitochondrial beta-oxidation, resulting in mitochondrial dysfunction, a reduction in ATP production, an imbalanced NADP+/NADPhydrogen(H) ratio, increased reactive oxygen species, and therefore apoptosis. Reduced exogenous fatty acids by depleting lipid or lowering serum supplementation exacerbated both shRNA depletion and pharmacological inhibition of ACACA-induced apoptosis in vitro. Collectively, our results suggest that inhibition of ectopic ACACA, together with suppression of exogenous fatty acid uptake, can be a novel strategy for treating currently incurable CRPC.