Changes in nonpolar aldehydes in bean cotyledons during ageing

• Published in: Biologia plantarum Vol. 50; no. 4; pp. 559 - 564
• Main Authors: Wilhelmova, N, Domingues, P.M.D.N, Srbova, M, Fuksova, H, Wilhelm, J
• Format: Journal Article
• Language: English
• Published: 01.12.2006
• Subjects: AGE; AGING; ALDEHIDOS; ALDEHYDE; ALDEHYDES; COTILEDONES; COTYLEDON; COTYLEDONS; EDAD; ENVEJECIMIENTO; ESPECTROMETRIA DE MASAS; FLUORESCENCE; FLUORESCENCIA; FREE RADICALS; FRIJOL (PHASEOLUS); HARICOT COMMUN; HPLC; http://www.fao.org/aos/agrovoc#c_10935; http://www.fao.org/aos/agrovoc#c_12421; http://www.fao.org/aos/agrovoc#c_186; http://www.fao.org/aos/agrovoc#c_192; http://www.fao.org/aos/agrovoc#c_1930; http://www.fao.org/aos/agrovoc#c_24469; http://www.fao.org/aos/agrovoc#c_250; http://www.fao.org/aos/agrovoc#c_33488; http://www.fao.org/aos/agrovoc#c_34043; http://www.fao.org/aos/agrovoc#c_4098; http://www.fao.org/aos/agrovoc#c_5472; http://www.fao.org/aos/agrovoc#c_5767; http://www.fao.org/aos/agrovoc#c_5875; KIDNEY BEANS; LIPID PEROXIDATION; MASS SPECTROMETRY; OXIDACION; OXIDATION; OXYDATION; PEROXIDACION LIPIDICA; PEROXYDATION DES LIPIDES; PHASEOLUS VULGARIS; PIGMENT; PIGMENTOS; PIGMENTS; RADICAL LIBRE; RADICALES LIBRES; SPECTROMETRIE DE MASSE; VIEILLISSEMENT
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/s10535-006-0088-z

Ageing of plant organs is accompanied by an increased production of free radicals what results in membrane lipid peroxidation. Non-polar aldehydes originating from this process interact with the cellular material to form the fluorescent end-products, lipofuscin-like pigments (LFP). Their formation was studied both qualitatively and quantitatively in ageing of bean cotyledons. The concentration of lipofuscin-like pigments increased 9-fold in 14-d-old (senescent) cotyledons in relation to 8-d-old (young) cotyledons. HPLC fractionation patterns indicate changes in their composition during ageing. The LFP increase in old cotyledons was accompanied by elevated levels of non-polar aldehydes that increased during ageing to 167%. The composition of aldehydes was studied by mass spectrometry. The most abundant fraction in both young and old cotyledon was represented by C12 aldehydes, which comprised both saturated and unsaturated species. We have observed differences in abundances of individual aldehydes between the young and the old cotyledons that might explain the differences in the composition of lipofuscin-like pigments. These results support the involvement of free radicals in plant ageing.