Tight Junction Protein 1 Suppresses Kidney Renal Clear Cell Carcinoma Cells Proliferation by Inducing Autophagy

TJP1, an adaptor protein of the adhesive barrier, has been found to exhibit distinct oncogenic or tumor suppressor functions in a cell-type dependent manner. However, the role of TJP1 in kidney renal clear cell carcinoma (KIRC) remains to be explored. The results showed a marked down-regulation of T...

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Published inInternational journal of medical sciences Vol. 20; no. 11; pp. 1448 - 1459
Main Authors Li, Miao, Zhou, Di-Sheng, Shao, Xin-Rong, Chan, Sze-Hoi, Dong, Zhao-Xia, Liu, Xue-Qi, Chen, Shu-Na, Qi, Lin, Zarate, Luis Velasquez, Wang, Xiao-Mei, Du, Xin, Zhang, Xing-Ding
Format Journal Article
LanguageEnglish
Published Wyoming Ivyspring International Publisher Pty Ltd 01.01.2023
Ivyspring International Publisher
Subjects
Antibodies
Autophagy
Bladder cancer
Cell culture
Cell growth
Cloning
Kidney cancer
Kinases
Medical prognosis
Proteins
Research Paper
Signal transduction
Survival analysis
Tumors
Variance analysis
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Summary:TJP1, an adaptor protein of the adhesive barrier, has been found to exhibit distinct oncogenic or tumor suppressor functions in a cell-type dependent manner. However, the role of TJP1 in kidney renal clear cell carcinoma (KIRC) remains to be explored. The results showed a marked down-regulation of TJP1 in KIRC tissues compared to normal tissues. Low expression of TJP1 was significantly associated with high grade and poor prognosis in KIRC. Autophagosome aggregation and LC3 II conversion demonstrated that TJP1 may induce autophagy signaling in 786-O and OS-RC-2 cells. Knockdown of TJP1 led to a decrease in the expression of autophagy-related genes, such as BECN1, ATG3, and ATG7. Consistently, TJP1 expression showed a significant positive correlation with these autophagy-related genes in KIRC patients. Furthermore, the overall survival analysis of KIRC patients based on the expression of autophagy-related genes revealed that most of these genes were associated with a good prognosis. TJP1 overexpression significantly suppressed cell proliferation and tumor growth in 786-O cells, whereas the addition of an autophagy inhibitor diminished its inhibitory function. Taken together, these results suggest that TJP1 serves as a favorable prognostic marker and induces autophagy to suppress cell proliferation and tumor growth in KIRC.
Bibliography:These authors contributed equally to this work.
Competing Interests: The authors have declared that no competing interest exists.
ISSN:1449-1907
1449-1907
DOI:10.7150/ijms.81065