cDNA sequence and deduced amino acid sequence of human preprocolipase

• Published in: Pancreas Vol. 6; no. 2; p. 157
• Main Authors: Renaud, W, Dagorn, J C
• Format: Journal Article
• Language: English
• Published: United States 01.03.1991
• Subjects: Amino Acid Sequence; Base Sequence; Blotting, Northern; Colipases - chemistry; Colipases - genetics; DNA - genetics; Enzyme Precursors - chemistry; Enzyme Precursors - genetics; Gene Library; Humans; Molecular Sequence Data; RNA, Messenger - genetics
• ISSN: 0885-3177
• DOI: 10.1097/00006676-199103000-00005

Complementary DNA clones for human pancreatic colipase were identified in human pancreatic cDNA libraries by hybridization with a pool of synthetic oligonucleotides containing all possible coding sequences for amino acids 75 to 80 of the partial human colipase protein sequence (Sternby, et al. Biochim Biophys Acta 1984;784:75). Alignment of overlapping cDNA clones yielded an mRNA sequence of 504 nucleotides [not including the poly(A) tail] encoding a polypeptide of 112 amino acids. The prepeptide comprised 17 amino acids, with an amino-terminal cluster of charged residues followed by a hydrophobic core of 12 residues typical of leader sequences. The deduced human procolipase sequence comprised 95 residues, including a propeptide of 5 residues. It was in complete agreement with the partial sequence previously obtained by protein sequencing. Northern blot analysis revealed that the polyadenylated preprocolipase transcript had a length of approximately 680 nucleotides.