Green Synthesis of Silver Nanoparticles with Extracts from Kalanchoe fedtschenkoi : Characterization and Bioactivities

• Published in: Biomolecules (Basel, Switzerland) Vol. 14; no. 7; p. 782
• Main Authors: Mejía-Méndez, Jorge L, Sánchez-Ante, Gildardo, Cerro-López, Mónica, Minutti-Calva, Yulianna, Navarro-López, Diego E, Lozada-Ramírez, J Daniel, Bach, Horacio, López-Mena, Edgar R, Sánchez-Arreola, Eugenio
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 30.06.2024
• Subjects: Antibacterial activity; Antimicrobial agents; Antioxidants; Bacteria; Cancer; Chloroform; Disease; E coli; Electron microscopes; Enzymes; Flavonoids; Fourier transforms; Gram-negative bacteria; Gram-positive bacteria; green synthesis; Herbal medicine; Hydrogen peroxide; Kalanchoe fedtschenkoi; Metabolism; Metabolites; Morphology; Nanoparticles; Nanotechnology; Oxidative stress; Phytochemicals; Plant extracts; Polyphenols; Silver; silver nanoparticles; Spectroscopy; Spectrum analysis; Zeta potential; United States > US; nanotechnology; Kalanchoe fedtschenkoi; silver nanoparticles; green synthesis
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom14070782

In this work, the hexane, chloroform, and methanol extracts from were utilized to green-synthesize silver nanoparticles (Kf -, Kf -, and Kf -AgNPs). The Kf -, Kf -, and Kf -AgNPs were characterized by spectroscopy and microscopy techniques. The antibacterial activity of AgNPs was studied against bacteria strains, utilizing the microdilution assay. The DPPH and H O assays were considered to assess the antioxidant activity of AgNPs. The results revealed that Kf -, Kf -, and Kf -AgNPs exhibit an average diameter of 39.9, 111, and 42 nm, respectively. The calculated ζ-potential of Kf -, Kf -, and Kf -AgNPs were -20.5, -10.6, and -7.9 mV, respectively. The UV-vis analysis of the three samples demonstrated characteristic absorption bands within the range of 350-450 nm, which confirmed the formation of AgNPs. The FTIR analysis of AgNPs exhibited a series of bands from 3500 to 750 cm , related to the presence of extracts on their surfaces. SEM observations unveiled that Kf - and Kf -AgNPs adopted structural arrangements related to nano-popcorns and nanoflowers, whereas Kf -AgNPs were spherical in shape. It was determined that treatment with Kf -, Kf -, and Kf -AgNPs was demonstrated to inhibit the growth of , , and in a dose-dependent manner (50-300 μg/mL). Within the same range, treatment with Kf -, Kf -, and Kf -AgNPs decreased the generation of DPPH (IC 57.02-2.09 μg/mL) and H O (IC 3.15-3.45 μg/mL) radicals. This study highlights the importance of using inorganic nanomaterials to improve the biological performance of plant extracts as an efficient nanotechnological approach.