Serum mir-31-5p is a reliable biomarker in patients with oral squamous cell carcinoma and oral lichen planus

• Published in: Egyptian Journal of Medical Human Genetics Vol. 25; no. 1; pp. 77 - 6
• Main Authors: Mohtasham, Nooshin, Ghorbani, Zahra, Ayatollahi, Hossein, Arab, Fatemeh, Aghaee-Bakhtiari, Seyed Hamid, Rasoulian, Bashir, Mohajertehran, Farnaz
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 05.07.2024; Springer Nature B.V; SpringerOpen
• Subjects: Biomarkers; Biopsy; Chromosome 5; Dentistry; Diagnosis; Gene expression; Lichen planus; Medical prognosis; Medicine; Medicine & Public Health; Metastasis; MicroRNAs; miRNA; Oral cancer; Oral carcinoma; Oral lichen planus; Oral squamous cell carcinoma; qRT-PCR; Serum microRNA; Squamous cell carcinoma; Statistical analysis; Tumors; Iran; United States > US; Oral lichen planus; Oral squamous cell carcinoma; qRT-PCR; Serum microRNA
• ISSN: 1110-8630; 2090-2441
• DOI: 10.1186/s43042-024-00531-4

Background MicroRNAs have been proposed as a novel regulatory biomarker for gene expression and early diagnosis of cancers. In this study, we evaluate the expression level of miR-31-5p in the serum of patients with oral squamous cell carcinoma, oral lichen planus, and a healthy control group to obtain a primary diagnostic biomarker. Materials and methods Serum was collected from patients with oral lichen planus ( n  = 32), patients with oral squamous cell carcinoma ( n  = 35), and healthy subjects ( n  = 32). MicroRNA was isolated from serum and cDNA was made from it. Then, the quantitative and qualitative expression of miR-31-5p levels among the samples was checked by the qRT-PCR method. Results In this study, three groups were quantitatively and qualitatively analyzed for miR-31-5p expression in serum. The results showed that there was a statistically significant correlation between the mean quantitative and qualitative expression of miR-31-5p among the three groups ( P  < 0.05). Conclusions The expression of miR-31-5p was significantly higher in patients with oral squamous cell carcinoma and oral lichen planus compared with healthy controls. MiR-31-5p can be considered as a biomarker in serum that could be potentially reliable in the diagnosis and prognosis of oral squamous cell carcinoma and also in the transformation of lichen planus.