Automated Analysis of Fluorescence Colocalization: Application to Mitophagy

Mitophagy is a peculiar form of organelle-specific autophagy that targets mitochondria. This process ensures cellular homeostasis, as it fosters the disposal of aged and damaged mitochondria that otherwise would be prone to produce reactive oxygen species and hence endanger genomic stability. Simila...

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Published inMethods in enzymology Vol. 588; p. 219
Main Authors Sauvat, A, Zhou, H, Leduc, M, Gomes-da-Silva, L C, Bezu, L, Müller, K, Forveille, S, Liu, P, Zhao, L, Kroemer, G, Kepp, O
Format Journal Article
LanguageEnglish
Published United States 2017
Subjects
Cell Culture Techniques - methods
Cell Line, Tumor
Humans
Microscopy, Fluorescence - methods
Mitochondria - metabolism
Mitochondria - ultrastructure
Mitochondrial Degradation
Optical Imaging - methods
Protein Transport
Ubiquitin-Protein Ligases - analysis
Ubiquitin-Protein Ligases - metabolism
High content
Image analysis
Autophagy
Biosensor
Parkin
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Summary:Mitophagy is a peculiar form of organelle-specific autophagy that targets mitochondria. This process ensures cellular homeostasis, as it fosters the disposal of aged and damaged mitochondria that otherwise would be prone to produce reactive oxygen species and hence endanger genomic stability. Similarly, autophagic clearance of depolarized mitochondria plays a fundamental role in organismal homeostasis as exemplified by the link between Parkinson disease and impaired function of the mitophagy-mediating proteins PINK1 and Parkin. Here, we detail an image-based approach for the quantification of mitochondrial Parkin translocation, which is mechanistically important for the initiation of mitophagy.
ISSN:1557-7988
DOI:10.1016/bs.mie.2016.09.082