UV-B response of green and etiolated barley seedlings

• Published in: Biologia plantarum Vol. 51; no. 4; pp. 699 - 706
• Main Authors: Fedina, I.,Bulgarian Academy of Sciences, Sofia (Bulgaria). Acad. M. Popov Inst. of Plant Physiology, Velitchkova, M.,Bulgarian Academy of Sciences, Sofia (Bulgaria). Inst. of Biophysics, Georgieva, K.,Bulgarian Academy of Sciences, Sofia (Bulgaria). Acad. M. Popov Inst. of Plant Physiology, Demirevska, K.,Bulgarian Academy of Sciences, Sofia (Bulgaria). Acad. M. Popov Inst. of Plant Physiology, Simova, L.,Bulgarian Academy of Sciences, Sofia (Bulgaria). Acad. M. Popov Inst. of Plant Physiology
• Format: Journal Article
• Language: English
• Published: 01.12.2007
• Subjects: ACTIVIDAD ENZIMATICA; ACTIVITE ENZYMATIQUE; AHILAMIENTO; BARLEY; CAROTENOIDE; CAROTENOIDES; CAROTENOIDS; CATALASA; CATALASE; CEBADA; CHLOROPHYLLE; CHLOROPHYLLS; CLOROFILAS; DARKNESS; ELECTROFORESIS; ELECTROPHORESE; ELECTROPHORESIS; ENZYME ACTIVITY; ETIOLATION; ETIOLEMENT; FLAVONOIDE; FLAVONOIDES; FLAVONOIDS; FLUORESCENCE; FLUORESCENCIA; HORDEUM VULGARE; http://www.fao.org/aos/agrovoc#c_10935; http://www.fao.org/aos/agrovoc#c_13251; http://www.fao.org/aos/agrovoc#c_1333; http://www.fao.org/aos/agrovoc#c_1380; http://www.fao.org/aos/agrovoc#c_15706; http://www.fao.org/aos/agrovoc#c_1577; http://www.fao.org/aos/agrovoc#c_15987; http://www.fao.org/aos/agrovoc#c_16123; http://www.fao.org/aos/agrovoc#c_2124; http://www.fao.org/aos/agrovoc#c_24091; http://www.fao.org/aos/agrovoc#c_2523; http://www.fao.org/aos/agrovoc#c_2604; http://www.fao.org/aos/agrovoc#c_2964; http://www.fao.org/aos/agrovoc#c_33542; http://www.fao.org/aos/agrovoc#c_3662; http://www.fao.org/aos/agrovoc#c_3725; http://www.fao.org/aos/agrovoc#c_4322; http://www.fao.org/aos/agrovoc#c_5477; http://www.fao.org/aos/agrovoc#c_5691; http://www.fao.org/aos/agrovoc#c_6223; http://www.fao.org/aos/agrovoc#c_6945; http://www.fao.org/aos/agrovoc#c_8046; http://www.fao.org/aos/agrovoc#c_823; HYDROGEN PEROXIDE; ISOENZIMAS; ISOENZYME; ISOENZYMES; LIGHT; LIGHT REGIMES; LUMIERE; LUZ; MALONDIALDEHYDE; OBSCURIDAD; OBSCURITE; ORGE; OXIGENO; OXYGEN; OXYGENE; PEPTIDE; PEPTIDES; PEPTIDOS; PEROXIDASAS; PEROXIDASES; PEROXIDO DE HIDROGENO; PEROXYDASE; PEROXYDE D'HYDROGENE; PLANTULAS; PLANTULE; PROLINA; PROLINE; RADIACION ULTRAVIOLETA; RAYONNEMENT ULTRAVIOLET; REGIME LUMINEUX; REGIMENES DE LUZ; RUBISCO; SEEDLINGS; SUPEROXIDE DISMUTASE; SUPEROXIDO DISMUTASA; SUPEROXYDE DISMUTASE; ULTRAVIOLET RADIATION
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/s10535-007-0145-2

Seven-d-old etiolated and green barley seedlings (Hordeum vulgare cv. Alfa) were irradiated with UV-B for 30 min and then kept for 24 h in light or darkness. Chlorophyll (Chl) synthesis was inhibited by about 30% as a result of UV-B irradiation, but there were no significant changes in photochemical activity measured by variable to maximum fluorescence ratio (Fv/Fm), quantum yield and oxygen evolution rate. Electron transport of etiolated seedlings was similar to that of green ones, nevertheless, the Chl content was more then 2-fold lower. Ribulose-1,5-bisphosphate carboxylase/oxygenase large and small subunits were diminished as a result of UV-B irradiation in both etiolated and green plants, especially in those kept in the darkness. Catalase activity decreased and total superoxide dismutase activity increased in both types of plants following UV-B treatment. When benzidine was used as a substrate, an isoform located between guaiacol peroxidases 2 and 3 (guaiacol peroxidase X) appeared, which was specific for UV-B treatment. As a result of irradiation, the contents of UV-B absorbing and UV-B induced compounds increased in green seedlings but not in etiolated ones.