Establishment and Characterization of Human Glioblastoma Cell Line (HUBT‐n)

• Published in: Human cell : official journal of Human Cell Research Society Vol. 18; no. 1; pp. 59 - 65
• Main Authors: ISHWATA, Isamu, ISHIWATA, Chieko, ISHWATA, Emiko, IGUCHI, Megumi, SATO, Yoshiro, SONOBE, Makoto, KIGUCHI, Kazushige, TACHIBANA, Toshiaki, ISHIKAWA, Hiroshi
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.03.2005
• Subjects: Aged; Animals; biological characteristic; Brain Neoplasms - genetics; Brain Neoplasms - metabolism; Brain Neoplasms - pathology; Brain Neoplasms - ultrastructure; cell line; Cell Line, Tumor; Cell Proliferation; Female; glioblastoma; Glioblastoma - genetics; Glioblastoma - metabolism; Glioblastoma - pathology; Glioblastoma - ultrastructure; Humans; Karyotyping; Male; Mice; Mice, Inbred BALB C; Mice, Nude; Microscopy, Electron, Transmission; Neoplasm Transplantation
• ISSN: 0914-7470; 1749-0774
• DOI: 10.1111/j.1749-0774.2005.tb00058.x

We succeeded in primary culture of 3 in 4 cases of glioblastomas. The longterm passage cultures were not done from the primary cultures of original tumor, but glioblastoma cell line (HUBT‐n) was established from a xenograft of nude mouse. This line grew well without interruption for 4 years and was subcultivated over 120 times. The cells were spindle like or round in shape and neoplastic and pleomorphic features contained glial fibrillar acid protein (GFAP) and S l O O protein and grew multilayering without contact inhibition. A bough‐shaped long projection was noted from a small cell. One of the characteristics of the HUBT‐n cells was existence of well of nude mouse and produced the tumor resembling the original tumor.