High-sensitivity immunoassay on interdigitated electrode to detect osteoporosis biological marker

• Published in: Biotechnology and applied biochemistry Vol. 71; no. 2; p. 256
• Main Authors: Liu, Jianwen, Chen, Xiaohai, Lv, Hanlin, He, Shuming, Fan, Yong
• Format: Journal Article
• Language: English
• Published: United States 01.04.2024
• Subjects: gold; nanostructure; biosensor; clinical marker; silica
• ISSN: 1470-8744
• DOI: 10.1002/bab.2537

Osteoporosis is with porous bones, which refers to a decrease in the bone mineral density and weakens the bones to become brittle. Osteoporosis often progresses without any pain or symptoms until the bone fractures. Monitoring the condition of bone regularly helps to identify the bone that weakens at its earlier stages. In general, radiological techniques have been used to measure bone mineral density, are expensive, and the procedures are complicated. Therefore, researchers are focusing on the alternative method of biomarker quantification to identify bone mineral density. This research work was focused on quantifying the osteoporosis biomarker of C-terminal telopeptide of type I collagen (CTX-I) on an interdigitated electrode (IDE) sensor. Gold nanomaterial-modified anti-CTX-I antibody was attached to silica nanomaterial-decorated IDE and then identified by CTX-I interaction. Higher immobilization of antibodies was recorded on diamond-modified IDE through gold nanoparticles, and detected CTX-I as low as 0.5 pg/mL [y = 1.5507x - 0.9043 R = 0.9715], determined on a linear curve at the range 0.5-3.5 ng/mL. Further, specific identification of CTX-I was confirmed by control performances with osteopontin, IL-6, and anti-IgG antibody.