Oxidized high-density lipoprotein promotes maturation and migration of bone marrow derived dendritic cells from C57BL/6J mice

• Published in: Chinese medical sciences journal Vol. 23; no. 4; pp. 224 - 229
• Main Authors: Xu, Zeng-xiang, Yang, Yong-zong, Feng, Da-ming, Wang, Shuang, Tang, Ya-ling, He, Fan, Xia, Yan, Li, Fang
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.12.2008; Department of Pathology,Wannan Medical College,Wuhu,Anhui 241000; Institute of Cardiovascular Diseases,University of South China,Hengyang,Hunan 421001%Institute of Cardiovascular Diseases,University of South China,Hengyang,Hunan 421001
• Subjects: Animals; atherosclerosis; Bone Marrow Cells - cytology; Bone Marrow Cells - drug effects; Bone Marrow Cells - physiology; Cell Differentiation - drug effects; Cell Movement - drug effects; Cells, Cultured; dendritic cell; Dendritic Cells - cytology; Dendritic Cells - drug effects; Dendritic Cells - physiology; Humans; Lipoproteins, HDL - metabolism; Lipoproteins, HDL - pharmacology; Lipoproteins, LDL - metabolism; Lipoproteins, LDL - pharmacology; maturation; Mice; Mice, Inbred C57BL; migration; oxidized high-density lipoprotein; atherosclerosis; migration; maturation; oxidized high-density lipoprotein; dendritic cell
• ISSN: 1001-9294
• DOI: 10.1016/S1001-9294(09)60043-2

To explore the influence of oxidized high-density lipoprotein (oxHDL) on the maturation and migration of bone marrow-derived dendritic cells (BMDCs) from C57BL/6J mice. The C57BL/6J mice bone marrow cell suspension was prepared and purified. Recombinant granulocyte-macrophage colony-stimulating factor (rmGM-CSF) and recombinant interleukin-4 (rmIL-4) were used to promote monocytes to differentiate and suppress lymphocytes. Then 50 μg/mL oxHDL was added to stimulate BMDCs, using 50 μg/mL high-density lipoprotein (HDL) as homologous protein control, PBS as negative control, and 1 ng/mL lipopolysaccharide (LPS) as positive control. The CD86 and MHCII expression rates were detected with fluorescence-activated cell sorting (FACS). Liquid scintillation counting (LSC) was used in mixed lymphocyte reactions (MLRs) to reflect the ability of BMDCs in stimulating the proliferation of homologous T cells. Levels of cytokines IL-12 and IL-10 were detected by ELISA. The cell migration was evaluated with the transwell system. Compared with PBS group, the expressions of CD86 and MHCII, counts per minute of MLRs, secretion of IL-12 and IL-10, and number of migrated cells in oxHDL group and LPS group significantly increased (allP< 0.05), while the increment was less in oxHDL group than LPS group. The number of migrated cells in oxHDL group was about twice of that in HDL group. OxHDL may promote the maturation and migration of BMDCs in vitro.