Molecular Analysis of Genes CEBPA, NPM1, IDH1, and RUNX1 Polymorphisms as Biomarker Potential in Leukemia Patients

• Published in: Molecular carcinogenesis Vol. 64; no. 2; pp. 357 - 368
• Main Authors: Bashir, Kashif, Ghafar, Sadia Abdul, Rehman, Afifa Tur, Waris, Tayyaba, Farooq, Fatima, Alamin, Amin A.
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.02.2025
• Subjects: Adolescent; Adult; Aged; biomarker potential; Biomarkers; Biomarkers, Tumor - genetics; Case-Control Studies; CCAAT-Enhancer-Binding Proteins - genetics; CEBPA; Core Binding Factor Alpha 2 Subunit - genetics; Female; Genes; Genetic Predisposition to Disease; Genotype; Heterozygotes; Humans; IDH1, and RUNX1 genes; Isocitrate Dehydrogenase - genetics; Leukemia; Leukemia - genetics; Male; Middle Aged; Mutants; NPM1; Nuclear Proteins - genetics; Nucleophosmin; Pakistan; Polymorphism, Single Nucleotide; polymorphisms; Runx1 protein; Single-nucleotide polymorphism; Young Adult; CEBPA; IDH1, and RUNX1 genes; leukemia; NPM1; Pakistan; biomarker potential; polymorphisms
• ISSN: 0899-1987; 1098-2744; 1098-2744
• DOI: 10.1002/mc.23846

ABSTRACT Leukemia is found in approximately 2.3 million people worldwide and causes many deaths all over the world. This research study was conducted to figure out the link of single nucleotide polymorphisms of genes CEBPA (rs34529039), NPM1 (rs753788683), IDH1 (of rs11554137) and RUNX1 (rs13051066) polymorphisms as biomarker potential in leukemia patients. A total of 600 subjects were included in the study which included 300 patients and 300 healthy controls with age and gender matched. After DNA extraction, PCR was carried out to analyze polymorphisms of selected genes. A significant association with increased risk of leukemia by almost twofolds is observed in homozygous mutant (AA) of rs34529039 SNP of gene CEBPA (odds ratio [OR] = 1.71; 95% confidence interval [CI] = 1.04–2.82; p = 0.03) while highly significant association but with decrease risk of leukemia is observed in heterozygote genotype (CA) of same SNP (OR = 0.36; 95% CI = 0.22–0.59; p = 0.0001). A highly significant association with increased risk of leukemia up to twofolds is observed in heterozygote genotype (AG) of rs753788683 of gene NPM1 (OR 2.10: 95% CI 1.32–3.36 p = 0.0017) while increasing risk by two‐fold and show significant association in homozygous mutant (AA) (OR = 1.75; 95% Cl = 1.09–2.79; p = 0.01). Leukemia risk increases by twofold and shows significant association in the homozygous mutant (AA) of rs11554137 (OR = 1.75; 95%Cl = 1.09–2.79; p = 0.01). Leukemia risk increases by twofold and shows significant association in the homozygous mutant (AA) of rs13051066 of gene RUNX1 (OR = 0.63; 95%Cl = 0.39–1.63; p = 0.06).