Molecular identification of Bambusa changningensis is the natural bamboo hybrid of B. rigida × Dendrocalamus farinosus
Bamboo is one of the fastest-growing plants commonly used in food, fibre, paper, biofuel, ornamental and medicinal industries. Natural hybridization in bamboo is rare due to its long vegetative period followed by gregarious flowering and death of the entire population. In the current study, a new ba...
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Published in | Frontiers in plant science Vol. 14; p. 1231940 |
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Main Authors | , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
01.09.2023
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Subjects | |
Online Access | Get full text |
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Summary: | Bamboo is one of the fastest-growing plants commonly used in food, fibre, paper, biofuel, ornamental and medicinal industries. Natural hybridization in bamboo is rare due to its long vegetative period followed by gregarious flowering and death of the entire population. In the current study, a new bamboo species,
Bambusa changningensis
, shows intermediate characteristics of
Dendrocalamus farinosus
and
B. rigida
morphologically, but it is unknown whether
B. changningensis
is a natural hybrid. Moreover,
B. changningensis
has been identified as a superior variety of Sichuan Province with high pulping yield, fibre length and width. Therefore, we analyzed the morphological characteristics, DNA markers, DNA barcoding and chloroplast genomes to identify the hybrid origin of
B. changningensis
and possible maternal parent. We have developed the transcriptomic data for
B. changningensis
and mined the SSR loci. The putative parental lines and hybrid were screened for 64 SSR makers and identified that SSR14, SSR28, SSR31 and SSR34 markers showed both alleles of the parental species in
B. changningensis
, proving heterozygosity. Sequencing nuclear gene
GBSSI
partial regions and phylogenetic analysis also confirm the hybrid nature of
B. changningensis
. Further, we have generated the complete chloroplast genome sequence (139505 bp) of
B. changningensis
. By analyzing the cp genomes of both parents and
B. changningensis
, we identified that
B. rigida
might be the female parent. In conclusion, our study identified that
B. changningensis
is a natural hybrid, providing evidence for bamboo’s natural hybridization. This is the first report on confirming a natural bamboo hybrid and its parents through SSR and chloroplast genome sequence. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Eduardo Ruiz-Sanchez, University of Guadalajara, Mexico; Xiaoxuan Tian, Tianjin University of Traditional Chinese Medicine, China; Diaga Diouf, Cheikh Anta Diop University, Senegal These authors have contributed equally to this work Edited by: Christopher John Lambrides, The University of Queensland, Australia |
ISSN: | 1664-462X 1664-462X |
DOI: | 10.3389/fpls.2023.1231940 |