Changes in mixed lymphocyte culture-reactive lymphocytes following alloimmunization of single lymph nodes in sheep

When an "isolated" single lymph node is challenged with irradiated allogeneic lymphocytes, there is a change in the reactivity of the lymphocytes flowing out of the node when they are cultured in vitro in unidirectional mixed lymphocyte cultures (MLC) against the immunizing lymphocytes. Th...

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Bibliographic Details
Published inTransplantation Vol. 27; no. 2; p. 102
Main Authors Cahill, R N, Frost, H, Hay, J B, Lafleur, L, Trnka, Z
Format Journal Article
LanguageEnglish
Published United States 01.02.1979
Subjects
Animals
Cell Separation
Female
Kinetics
Lymph Nodes - immunology
Lymphocyte Culture Test, Mixed
Lymphocyte Transfusion
Lymphocytes - immunology
Male
Sheep - immunology
Transplantation, Homologous
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Summary:When an "isolated" single lymph node is challenged with irradiated allogeneic lymphocytes, there is a change in the reactivity of the lymphocytes flowing out of the node when they are cultured in vitro in unidirectional mixed lymphocyte cultures (MLC) against the immunizing lymphocytes. These changes in the reactivity of the recipient lymphocytes are shortlived, follow a set time sequence in relation to the cell traffic changes accompanying the immune response, are the property of small lymphocytes and not blast cells, are exhibited by surface Ig-negative cells, and they are specific for the donor lymphocytes. It is suggested that antigen causes the selective retention of antigen-specific lymphocytes within the stimulated node followed by the proliferation and differentiation of large numbers of antigen-specific cells, which then leave the lymph node as small lymphocytes.
ISSN:0041-1337
DOI:10.1097/00007890-197902000-00007