Lactobacillus silagei sp. nov., isolated from orchardgrass silage

• Published in: International journal of systematic and evolutionary microbiology Vol. 63; no. Pt 12; pp. 4613 - 4618
• Main Authors: Tohno, Masanori, Kitahara, Maki, Irisawa, Tomohiro, Masuda, Takaharu, Uegaki, Ryuichi, Ohkuma, Moriya, Tajima, Kiyoshi
• Format: Journal Article
• Language: English
• Published: England 01.12.2013
• Subjects: Bacterial Typing Techniques; Base Composition; Dactylis - microbiology; Dactylis glomerata; DNA, Bacterial - genetics; Fatty Acids - chemistry; Japan; Lactobacillus; Lactobacillus - classification; Lactobacillus - genetics; Lactobacillus - isolation & purification; Molecular Sequence Data; Nucleic Acid Hybridization; Peptidoglycan - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Silage - microbiology; Japan
• ISSN: 1466-5026; 1466-5034; 1466-5034
• DOI: 10.1099/ijs.0.053124-0

A Gram-reaction-positive, facultatively anaerobic, non-spore-forming and catalase-negative rod-shaped bacterial strain, designated IWT126(T), was isolated from orchardgrass (Dactylis glomerata L.) silage preserved in Hachimantai, Iwate, Japan. The isolate showed growth at 15-45 °C, pH 3.5-7.5 and with 4.0 % (w/v) NaCl. The cell wall peptidoglycan did not contain meso-diaminopimelic acid, and the DNA G+C content was 45.6 mol%. The major cellular fatty acids were C16 : 0 and C19 : 1 cyclo 9,10. Based on 16S rRNA gene sequence similarity, strain IWT126(T) was classified as a member of the genus Lactobacillus and was most closely related to Lactobacillus odoratitofui YIT 11304(T) (98.7 %), Lactobacillus similis JCM 2765(T) (98.5 %), Lactobacillus collinoides JCM 1123(T) (97.6 %), Lactobacillus paracollinoides DSM 15502(T) (97.6 %) and Lactobacillus kimchicus DCY51(T) (96.9 %). Based on sequence analysis of the phenylalanyl-tRNA synthase α-subunit (pheS) gene, strain IWT126(T) was well separated from its phylogenetic neighbours in the genus Lactobacillus. Based on physiological, biochemical and genotypic results, as well as low DNA-DNA relatedness to recognized phylogenetic relatives in the genus Lactobacillus, classification of strain IWT126(T) as a representive of a novel species named Lactobacillus silagei sp. nov. is proposed. The type strain is IWT126(T) ( = JCM 19001(T) = DSM 27022(T)).