Automatic inoculation of bacterial cultures using low-melting point, wax-like coconut oil

A simple method for the automatic inoculation of broth culture tubes is described. An incubator is plugged into an electrical outlet timer. Heating of the incubator causes wax-like coconut oil to melt and allow an inoculum-coated bead to drop into sterile liquid medium. •Bacteria-coated bead sits at...

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Bibliographic Details
Published inJournal of microbiological methods Vol. 208; p. 106727
Main Author Ricca, David M.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 01.05.2023
Subjects
Automatic
Bacteria
Coconut Oil
Inoculation
Microbiological Techniques
Wax-like coconut oil
Bacteria
Inoculation
Wax-like coconut oil
Automatic
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Summary:A simple method for the automatic inoculation of broth culture tubes is described. An incubator is plugged into an electrical outlet timer. Heating of the incubator causes wax-like coconut oil to melt and allow an inoculum-coated bead to drop into sterile liquid medium. •Bacteria-coated bead sits atop wax-like layer of coconut oil above sterile growth medium.•Outlet timer turns on incubator.•Coconut oil melts, bead drops, and inoculates culture.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0167-7012
1872-8359
DOI:10.1016/j.mimet.2023.106727