Enrichment of gamma-linolenic acid from evening primrose oil and borage oil via lipase-catalyzed hydrolysis

• Published in: Journal of the American Oil Chemists' Society Vol. 71; no. 6; pp. 569 - 573
• Main Authors: Rahmatullah, M.S.K.S, Shukla, V.K.S, Mukherjee, K.D
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.06.1994
• Subjects: ACEITES VEGETALES; ACIDE LINOLENIQUE; ACIDO LINOLENICO; ACYLGLYCEROLS; Bioconversions. Hemisynthesis; Biological and medical sciences; Biotechnology; BORAGO OFFICINALIS; Fundamental and applied biological sciences. Psychology; GLICERIDOS; GLYCERIDE; GLYCERIDES; HIDROLISIS; HUILE VEGETALE; HYDROLYSE; HYDROLYSIS; LINOLENIC ACID; Methods. Procedures. Technologies; OENOTHERA BIENNIS; PLANT OILS; SEED OILS; TRIACILGLICEROL LIPASA; TRIACYLGLYCEROL LIPASE; Candida cylindracea; Enrichment; Yeast; Triacylglycerol lipase; Onagraceae; Esterases; Vegetal oil; Optimization; Fungi; Dicotyledones; Substrate specificity; Angiospermae; Oenothera biennis; Fungi Imperfecti; Thallophyta; Enzyme; Triglyceride; Boraginaceae; Carboxylic ester hydrolases; Hydrolysis; Gas chromatography; Organic solvent; Linolenic acid; Hydrolases; Spermatophyta; Borago officinalis; Enzymatic reaction
• ISSN: 0003-021X; 1558-9331
• DOI: 10.1007/BF02540580

Abstract Lipase‐catalyzed selective partial hydrolysis of evening primrose ( Oenothera biennis L.) seed oil and borage ( Borago officinalis L.) seed oil led to an increase in the level of γ‐linolenic acid (GLA; 18∶3n−6) in the unhydrolyzed acylglycerols. Thus, in evening primrose oil, the GLA level could be raised from 9.4% in the starting material to 46.5% in the unhydrolyzed acylglycerols by means of a lipase from Candida cylindracea . Selective hydrolysis of borage oil with Pancreatin led to an increase in the GLA content from 20.4% in the oil to 33.5% in the unhydrolyzed acylglycerols. Partial hydrolysis of borage oil with lipase from C. cylindracea raised the GLA content of the acylglycerols to 47.8%.