Characterization of the Fasciclin-like arabinogalactan gene family in Brassica napus and the negative regulatory role of BnFLA39 in response to clubroot disease stress

• Published in: Industrial crops and products Vol. 196; p. 116400
• Main Authors: Jia, Ruimin, Yu, Ligang, Chen, Jing, Hu, Lifang, Cao, Shang, Wang, Yang
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.06.2023
• Subjects: Brassica napus; Fasciclin-like arabinogalactan proteins; Gene expression profiling; Negative regulation; Plasmodiophora brassicae; TFs; GPI; DI; Gene expression profiling; Mw; Pb; CDS; MEME; Plasmodiophora brassicae; pI; Fasciclin-like arabinogalactan proteins; dpi; PBS; qRT-PCR; FAA; DAF; GO; AGP; CAEs; FLAs; Brassica napus; FAS; HRGPs; NJ; Negative regulation
• ISSN: 0926-6690; 1872-633X
• DOI: 10.1016/j.indcrop.2023.116400

Fasciclin-like arabinogalactan proteins (FLAs) are critical for regulation of plant development and environmental adaptation. Although FLAs have been reported in numerous plants, the FLAs family members in Brassica napus and their responses to plant pathogens are unknown. In this study, 59 BnFLAs identified from a genome-wide study were classified into 4 groups based on conserved motif compositions, gene structure analyses, and phylogenetic relationships. Furthermore, a number of stress-responsive and phytohormone responsive hormonal cis-regulatory elements were identified from BnFLAs, promoters, and may be the targets of transcription factors like ERF that are important in stress responses. Subcellular localization showed that most BnFLAs are localized in the cell membrane. Moreover, expression patterns at different growth stages showed that the BnFLAs are actively involved in B. napus growth and developmental processes. Expression analyses also revealed that BnFLAs respond significantly to salt and Plasmodiophora brassicae (Pb) stresses. In addition, a BnFLA39 homolog, AtFLA7, was shown to be a negative regulator of the defense responses of Arabidopsis to Pb stress. The protein interaction analysis revealed that BnFLA39 exerted biological functions by interacting with BnFLA15/43/26/53. This study provides the basis for further research on BnFLAs functions and regulatory mechanisms and provides insights for a better understanding of the roles of FLAs in Brassisca-Pb interactions. [Display omitted] •A total of 59 FLA genes were identified in the Brassica napus genome, which was divided into 4 groups.•BnFLA genes are expressed in various tissues with different developmental stages.•BnFLA genes response to various abiotic stresses and all BnFLA genes were inhibited under salt treatments.•BnFLA39 acts as a negative regulator of B. napus rolerance to clubroot disease stress.