The reaction of diazomethane with synthetic phosphatides and with insect lipid extracts

1. 1. The reaction of diazomethane with synthetic phosphatidylethanolamine, N-methylphosphatidylethanolamine, phosphatidylcholine and insect-lipid extracts was studied in order to find out if the reaction was specific to one type of phosphatide. 2. 2. The three synthetic phosphatides reacted in esse...

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Bibliographic Details
Published inBiochimica et biophysica acta Vol. 84; no. 6; pp. 665 - 680
Main Author Crone, H.D.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 02.12.1964
Subjects
Alkylation
Animals
Azo Compounds
Chemical Phenomena
Chemistry
Choline
Chromatography
Diazomethane
Insecta
Lipids
OldMedline
Phospholipids
Phosphorus Isotopes
Spectrum Analysis
CHOLINE
ALKYLATION
EXPERIMENTAL LAB STUDY
CHEMISTRY
INSECTS
PHOSPHOLIPIDS
PHOSPHORUS ISOTOPES
AZO COMPOUNDS
CHROMATOGRAPHY
SPECTRUM ANALYSIS
LIPIDS
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Summary:1. 1. The reaction of diazomethane with synthetic phosphatidylethanolamine, N-methylphosphatidylethanolamine, phosphatidylcholine and insect-lipid extracts was studied in order to find out if the reaction was specific to one type of phosphatide. 2. 2. The three synthetic phosphatides reacted in essentially the same manner, although with different rates of reaction, forming dimethyl esters of phosphatidic acids and a nitrogenous compound similar to neurine. The results with insect extracts gave evidence of the reaction of diazomethane with many natural phosphatides. 3. 3. N-methylation of the intact ethanolamine phosphatides occurred to a limited extent and was increased by the presnece of alcohols in the reaction medium. 4. 4. Techniques used included column chromatography on silicic acid, paper chromatography and infrared spectroscopy.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0926-6542
0006-3002
1878-2264
DOI:10.1016/0926-6542(64)90025-3