A putative secreted solute binding protein, SCO6569 is a possible AfsR2-dependent down-regulator of actinorhodin biosynthesis in Streptomyces coelicolor

• Published in: Process biochemistry (1991) Vol. 44; no. 3; pp. 373 - 377
• Main Authors: Lee, Han-Na, Im, Jong-Hyuk, Lee, Mi-Jin, Lee, Sang Yup, Kim, Eung-Soo
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 01.03.2009
• Subjects: Actinorhodin; AfsR2; Down-regulator; SCO6569; Streptomyces; Streptomyces; AfsR2; Down-regulator; SCO6569; Actinorhodin
• ISSN: 1359-5113; 1873-3298
• DOI: 10.1016/j.procbio.2008.12.002

AfsR2 is a global regulatory protein involved in stimulating the biosynthesis of antibiotics in various Streptomyces species. Using 2-D gel electrophoresis and MALDI-TOF analyses, several putative AfsR2-target proteins were previously identified. In order to verify the biological significance of the proteomics-guided targets, the sixteen putative AfsR2 target genes were individually cloned under the strong-and-constitutive promoter (PermE *) of the Streptomyces expression vector, pSE34, followed by the transformation into both Streptomyces lividans and Streptomyces coelicolor. Among the putative AfsR2-target genes tested, the expression of a putative secreted solute binding protein, SCO6569 significantly repressed biosynthesis of actinorhodin in S. coelicolor, and the transcripts encoded by the pathway-specific activator, actII-ORF4 for actinorhodin were also reduced in SCO6569-overexpressing S. coelicolor. Moreover, SCO6569 gene-disruption in S. coelicolor further enhanced actinorhodin production, suggesting that the SCO6569 could be a possible AfsR2-dependent down-regulator acting in actinorhodin biosynthesis in S. coelicolor.