Nematicidal, antifungal and insecticidal activities of Artemisia halodendron extracts: New polyacetylenes involved

• Published in: Industrial crops and products Vol. 170; p. 113825
• Main Authors: Wu, Hai-bo, Guo, Peng-xin, Ma, Lin-hui, Li, Xi-meng, Liu, Ting-ting
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 15.10.2021
• Subjects: Antifungal activity; Artemisia halodendron; Insecticidal activity; Microwave-assisted extraction; Nematicidal activity; Polyacetylenes; Microwave-assisted extraction; DMSO; TPC; GAEs; FADs; COSY; TFC; Antifungal activity; SFE; IC50; QEs; Polyacetylenes; CC; MIC; HMBC; SOE; Insecticidal activity; MAC; LD50; MAE; LC50; HR-ESI-MS; NMR; Artemisia halodendron; UAE; TPAC; Nematicidal activity
• ISSN: 0926-6690; 1872-633X
• DOI: 10.1016/j.indcrop.2021.113825

•Polyacetylenes extracted from Artemisia halodendron showed a strong biopesticidal effects.•Microwave-assisted extraction is the best method to extract polyacetylenes.•Two new and four known polyacetylenes were isolated by the bioassay-guided method.•Nematicidal and insecticidal activities of polyacetylenes 1–4 were higher than that of positive control. The present study evaluates the nematicidal, antifungal and insecticidal effects of extracts from different organs (leaves, stems and roots) of Artemisia halodendron Turcz. ex Bess. Correlative analysis revealed that polyacetylenes were the main contributors to biological activities. Furthermore, five extraction techniques were compared and evaluated based on the yield of polyacetylenes and their activities. It was found that microwave-assisted extraction (MAE) was the best extracting approach. Additionally, two new polyacetylenes (1–2) and four known polyacetylenes (3–6) were isolated from the MAE extract. All polyacetylenes showed remarkable nematicidal effect against Meloidogyne incognita (Kofoid & White) Chitwood (50 % lethal concentration, LC50, 0.21–9.46 mg/L) compared with abamectin (LC50, 9.98 mg/L). In particular, nematicidal activity of the new compound 1 was 48-times higher than that of abamectin. Also, these polyacetylenes exhibited antifungal effects against five fungi (minimum inhibitory concentration, MIC, 4–128 mg/L). Moreover, polyacetylenes 1–4 showed remarkable insecticidal activity against Bradysia odoriphaga Yang & Zhang (50 % lethal dose, LD50, 19.31–36.51 mg/L), stronger than the azadirachtin (LD50, 39.30 mg/L). Together, the results gained from this study suggest that A. halodendron and the isolated polyacetylenes have great potential application in nematicide, fungicide and insecticide agent industry.