ApoE-modified liposomes encapsulating resveratrol and salidroside alleviate manifestations of Alzheimer's disease in APP/PS-1 mice

• Published in: Drug development and industrial pharmacy Vol. 49; no. 9; p. 559
• Main Authors: Chen, Mu-Han, Liu, Xin-Ze, Qu, Xiu-Wu, Guo, Rui-Bo, Zhang, Lu, Kong, Liang, Yu, Yang, Liu, Yang, Zang, Juan, Li, Xiu-Ying, Li, Xue-Tao
• Format: Journal Article
• Language: English
• Published: England 02.09.2023
• Subjects: Alzheimer Disease - drug therapy; Animals; Apolipoproteins E - pharmacology; Apolipoproteins E - therapeutic use; Blood-Brain Barrier; Glucosides; Liposomes - pharmacology; Mice; Neurodegenerative Diseases; Phenols; Resveratrol - pharmacology; blood-brain barrier; salidroside; ApoE; Alzheimer’s disease; liposomes; resveratrol
• ISSN: 1520-5762
• DOI: 10.1080/03639045.2023.2252062

Alzheimer's disease (AD) is a neurodegenerative disease that is associated with aging and is influenced by both genetic and environmental factors. Several studies and clinical trials have demonstrated that resveratrol (Res) and salidroside (Sal) are not only biologically safe but also influence AD biomarker trajectories. However, their clinical applications have been quite limited due to poor specificity, low solubility, and insufficient blood-brain barrier (BBB) penetration. Therefore, we developed a nano-drug delivery system in which Res and Sal were encapsulated in liposomes, which were surface-modified with ApoE (ApoE-Res/Sal-Lips) to compensate for these deficiencies. In this study, ApoE-Res/Sal-Lips were prepared using a standard thin-film hydration method for liposomes. Then, cellular uptake of the loaded liposomes was assessed using fluorescent staining assays. A BBB model was constructed to investigate the capacity of the liposomes to cross the BBB , and the ability of liposomes to target the brain was observed by imaging. In addition, the neuroprotective effects of the different liposome formulations in APP/PS-1 mice were evaluated by measuring the changes in levels of oxidative, anti-inflammatory, and anti-apoptotic factors in the mice brains. , ApoE-Res/Sal-Lips increased the uptake of Res and Sal by bEnd.3 and N2a cells, enhanced BBB penetration, and improved transport efficiency. , the ApoE-Res/Sal-Lips were found to alleviate AD pathological symptoms, reduce learning and memory impairments, and improve brain function. ApoE-Res/Sal-Lips provide a new method for the treatment of AD.