Indirect somatic embryogenesis on rare octoploid Asparagus breslerianus plants

• Published in: Scientia horticulturae Vol. 226; pp. 184 - 190
• Main Authors: Mousavizadeh, Seyyed Javad, Mashayekhi, Kambiz, Hassandokht, Mohammad Reza
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 19.12.2017
• Subjects: Ancymidol; In vitro; Ploidy level; Wild asparagus; Wild asparagus; Ploidy level; 2,4-D; Ancymidol; MS; In vitro; BA; NAA
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/j.scienta.2017.08.031

•A. breslerianus calli subjected to BA were green in color and friable in type, while on 2,4-D were yellow in color, either compact or friable in type.•2,4-D and BA induced embryogenic calli, subsequent globular, bipolar and mature embryos were developed.•Successful acclimatization can be utilized by maintaining plantlets on regeneration media until storage roots develop. Asparagus breslerianus Schultes & Schultes fil is considered a rare wild octoploid species, which needs preservation against livestock grazing and erosion. In vitro method, especially somatic embryogenesis, is a useful method to perform mass micropropagation of identical clones. In the present study, spear buds of Asparagus breslerianus were used to obtain in vitro somatic embryos and, subsequently turn them into plantlets. Calli were developed on callus induction media (MS+0.88μM BA+1.07μM NAA). Then, they were transferred for four weeks to somatic embryogenesis induction phase media with different concentrations of 2,4-D (0, 4.53, 9.05 and 13.57μM) and BA (0, 4.44, 8.88 and 13.32μM). 2,4-D and BA were eliminated from induced calli media in realization phase. As a result globular, bipolar and mature embryos were observed six weeks later. Calli with compact or friable structures either green or yellowish produced embryogenic calli. Once the mature embryos developed shoots, they were transferred to root initiated media consisting of MS +3.9μM Ancymidol and 1.07μM NAA. Two months later, storage roots were produced on in vitro well-regenerated plantlets. The survival of asparagus plantlets in the acclimation phase was improved by the development of storage roots.