Estrogen receptor cytochemistry by fluorescent estrogen

A recently synthesized fluorescein-labeled estrogen (17FE, 1-(N)-fluoresceinyl-estrone-thiosemicarbazone) interacts with estrogen-target cells like the native hormone and visualizes the uptake, transport, and distribution of estrogen in intact target cells. Moreover, estrogen binding sites are trace...

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Published inThe journal of histochemistry and cytochemistry Vol. 28; no. 10; pp. 1081 - 1088
Main Authors Nenci, I, Dandliker, WB, Meyers, CY, Marchetti, E, Marzola, A, Fabris, G
Format Journal Article
LanguageEnglish
Published Los Angeles, CA Histochemical Soc 01.10.1980
SAGE Publications
Subjects
Animals
Breast - metabolism
Breast Neoplasms - metabolism
Cell Line
Estrone - analogs & derivatives
Estrone - metabolism
Fluoresceins - metabolism
Fluorescent Dyes
Histocytochemistry
Humans
Mice
Rats
Receptors, Estrogen - metabolism
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Summary:A recently synthesized fluorescein-labeled estrogen (17FE, 1-(N)-fluoresceinyl-estrone-thiosemicarbazone) interacts with estrogen-target cells like the native hormone and visualizes the uptake, transport, and distribution of estrogen in intact target cells. Moreover, estrogen binding sites are traced by 17FE in cryostat sections of estrogen target tissues as well. Cell and tissue 17FE binding sites fulfill the accepted criteria for specific estrogen receptors (finite binding capacity, high affinity, steroid and tissue specificity). This fluorescent probe allows estrogen receptors to be studied in a wide variety of cell and tissue preparations under varying conditions of physiologic and pathophysiologic interest.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0022-1554
1551-5044
DOI:10.1177/28.10.7419900