Cytotoxicity and genotoxicity induced by aflatoxin B1, ochratoxin A, and their combination in cultured Vero cells

Aflatoxin B1 (AFB1) and ochratoxin A (OTA) are important food‐borne mycotoxins that have been implicated in human health. In this study, independent and combinative toxicities of AFB1 and OTA were tested in cultured monkey kidney Vero cells. The experiments reported here were conducted to evaluate t...

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Published inJournal of biochemical and molecular toxicology Vol. 24; no. 1; pp. 42 - 50
Main Authors Golli-Bennour, Emna El, Kouidhi, Bochra, Bouslimi, Amel, Abid-Essefi, Salwa, Hassen, Wafa, Bacha, Hassen
Format Journal Article
LanguageEnglish
Published Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.01.2010
Subjects
Aflatoxin B1
Aflatoxin B1 - toxicity
Algorithms
Animals
Apoptosis
Apoptosis - drug effects
Blotting, Western
Cell Survival - drug effects
Cercopithecus aethiops
Combinative Toxicity
Cytotoxicity
Cytotoxins - toxicity
DNA Fragmentation - drug effects
Dose-Response Relationship, Drug
Drug Synergism
Food Contamination
Inhibitory Concentration 50
Kidney - drug effects
Kidney - metabolism
Ochratoxin A
Ochratoxins - toxicity
Proto-Oncogene Proteins c-bcl-2 - metabolism
Tumor Suppressor Protein p53 - metabolism
Vero Cells
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Summary:Aflatoxin B1 (AFB1) and ochratoxin A (OTA) are important food‐borne mycotoxins that have been implicated in human health. In this study, independent and combinative toxicities of AFB1 and OTA were tested in cultured monkey kidney Vero cells. The experiments reported here were conducted to evaluate the effect of these toxins on cell viability followed by the determination of cell death pathways, using the quantification of DNA fragmentation and the expression of p53 and bcl‐2 protein levels. Our results showed that AFB1 and OTA caused a marked decrease of cell viability in a dose‐dependent manner. Under the same conditions, these mycotoxins increased fragmented DNA levels. In addition, p53 was activated in response to DNA damage and the expression of the antiapoptotic factor bcl‐2 decreased significantly. According to these data, AFB1 and OTA seemed to be involved in an apoptotic process. Moreover, combined AFB1 and OTA induced all the toxicities observed with the mycotoxins separately. Therefore, this combination was classified as an additive response of the two mycotoxins. © 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 24:42–50, 2010; Published online in Wiley InterScience (www.interscience.wiley.com). DOI 10.1002/jbt.20310
Bibliography:istex:D607F2D5F4FF46A907EACD643F16148FC3AC0F0B
ArticleID:JBT20310
Ministère Tunisien de l'Enseignement Supérieur, de la Recherche Scientifique et de la Technologie" through the "Laboratoire de Recherche sur les Substances Biologiquement Compatibles.
ark:/67375/WNG-8VHTM4Q1-Q
Emna El Golli-Bennour and Bochra Kouidhi contributed equally to this article.
Emna El Golli‐Bennour and Bochra Kouidhi contributed equally to this article.
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ISSN:1095-6670
1099-0461
1099-0461
DOI:10.1002/jbt.20310