Trafficking of Th1 Cells to Lung: A Role for Selectins and a P-Selectin Glycoprotein-1-Independent Ligand

• Published in: American journal of respiratory cell and molecular biology Vol. 30; no. 2; pp. 220 - 227
• Main Authors: Clark, Joan G, Mandac-Dy, Janis B, Dixon, Anne E, Madtes, David K, Burkhart, Kristin M, Harlan, John M, Bullard, Daniel C
• Format: Journal Article
• Language: English
• Published: United States Am Thoracic Soc 01.02.2004; American Thoracic Society
• Subjects: Adoptive Transfer; Animals; Antibodies, Monoclonal - metabolism; Cell Adhesion; Cell Movement - physiology; Cells, Cultured; Humans; Lung - cytology; Lung - immunology; Membrane Glycoproteins - metabolism; Mice; Mice, Inbred C57BL; Neuraminidase - metabolism; P-Selectin - metabolism; Protein Binding; Recombinant Fusion Proteins - metabolism; Selectins - metabolism; Th1 Cells - metabolism
• ISSN: 1044-1549; 1535-4989
• DOI: 10.1165/rcmb.2003-0208OC

Trafficking of lymphocytes to lung is a critical component of pulmonary immune defense and surveillance. Selectins, expressed on vascular endothelium, regulate T lymphocyte emigration into tissues, such as skin, but the role of the selectins in trafficking of T cells to lung has not been well characterized. Here, we used a model of lung inflammation induced by adoptive transfer of alloreactive Th1 cells to analyze the role of P- and E-selectin in Th1 cell trafficking to lung in vivo. We found that both P- and E-selectin play an important role in Th1 lymphocyte migration to lung. We confirmed that the Th1 cells express P-selectin glycoprotein ligand-1, which was functional in binding to P- and E-selectin in vitro. However, our studies reveal that a ligand distinct from P-selectin glycoprotein-1 also binds these selectins in vitro and appears to play a physiologic role in in vivo emigration of Th1 lymphocytes into the lung.