Calcitriol combined with fish oil in the diet of Chukar partridge can ameliorate laying performance by improving oviduct histomorphometry and functions

• Published in: Journal of animal physiology and animal nutrition Vol. 107; no. 5; pp. 1206 - 1215
• Main Authors: Abdollahi, Arman, Akhlaghi, Amir, Zamiri, Mohammad Javad, Kargar, Shahryar, Ansari Pirsaraei, Zarbakht, Nasrollahi, Banafsheh, Kamali, Younes
• Format: Journal Article
• Language: English
• Published: Germany Wiley Subscription Services, Inc 01.09.2023
• Subjects: Alectoris chukar; Birds; Body weight; Calcitriol; Calcium (blood); Cholesterol; Diameters; Egg production; Eggs; Erythrocytes; Females; Fish oils; Follicles; Histomorphometry; Leukocytes; Males; Oral administration; Oviduct; Oviposition; Reproductive system; Sex; Sex ratio; Uterus; ovarian follicles; oviduct; omega-3 PUFA; partridge breeder; vitamin D3
• ISSN: 0931-2439; 1439-0396
• DOI: 10.1111/jpn.13817

The effects of calcitriol (CT) and/or fish oil (FO) on performance, oviposition time, sex ratio and morphology of the reproductive system of laying Chukar partridges were studied. Female (n = 48) and male (n = 16) partridges were used in a completely randomised design using a 2 × 2 factorial arrangement and were randomly allocated to either of four experimental treatments with four cage replicates of three females and one male each. Female birds received no FO (CON - FO) or were orally administered with 0.2 mL (0.24 g)/500 g body weight FO (CON + FO) or 0.2 mL solution containing 10 µg CT (CT - FO), or their combination (CT + FO) for 42 successive days. The eggs were collected every two hours between 07:00 and 23:00 h. Administering FO along with CT had considerably increasing effect on the male-biased sex ratio. FO and CT administration interacted to increase serum calcium concentration. Experimental treatments increased the number of leucocytes and erythrocytes. Serum cholesterol was decreased in CON + FO partridges compared with those of the CT - FO and CON - FO birds. There was an interaction between FO and CT on the weight of eggs and hatchlings, number of medium white follicles, diameter and the number of small yellow follicles, the weight and diameter of the fifth follicles (F5), and thickness of secondary mucosal folds in both uterus and vagina. Administering CT alone or with FO increased the feed intake, egg production, oviductal weight, diameter and number of large yellow follicles, the weight and diameter of the second (F2) and first (F1) follicles compared with those of the CON - FO females. Further studies are required to elucidate the mechanisms by which such changes in the sex ratio skew, ovary and oviduct are mediated.