Continuous free flow electrophoresis for preparative chiral separations of piperoxan using sulfated β-cyclodextrin

• Published in: Analyst (London) Vol. 125; no. 10; pp. 1719 - 1724
• Main Authors: STALCUP, Apryll M, SUTTON, Richard M. C, PAINULY, Prabha, VELA RODRIGO, Jesus, GRATZ, Samuel R, YANES, Enrique G
• Format: Journal Article
• Language: English
• Published: Cambridge Royal Society of Chemistry 01.01.2000
• Subjects: Adrenergic alpha-Antagonists - isolation & purification; Analysis; beta-Cyclodextrins; Biological and medical sciences; Cyclodextrins; Electrophoresis - methods; General pharmacology; Medical sciences; Pharmacology. Drug treatments; Piperoxan - isolation & purification; Stereoisomerism; Chemical analysis; Enantioselectivity; Capillary electrophoresis; Preparative chromatography; Oligoholoside; Macrocycle; Chiral auxiliary; Cyclodextrin; Chemical modification; Alpha blocking agent; Ultraviolet detector; α-Adrenergic receptor; Enantiomer; Piperoxan; Concentration effect; Qualitative analysis; Antagonist; Continuous flow method; Stationary phase
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/b004347h

Continuous free flow electrophoresis was investigated as a tool for the preparative chiral separation of piperoxan using a sulfated cyclodextrin chiral additive. In the absence of chiral additive, the sample stream was deflected cathodically. However, the presence of sulfated cyclodextrin in the run buffer caused anodic deflection and splitting of the sample stream into two streams, each enriched in one enantiomer. Although the sulfated cyclodextrin used was comprised of a mixture of homologues and isomers, this polydispersity did not seem to significantly impact band dispersion. Sample introduction rates ranged from approximately 0.9-7.2 mg h-1. Maximum resolution was 0.53, using an applied voltage of 220 V, buffer composition of 0.075% sulfated cyclodextrin, 7.6 mM citrate (pH 3), 4.5 degrees C.