Quantification of pentachlorophenol transformation product distribution in the presence of Phanerochaete chrysosporium

• Published in: Archives of environmental contamination and toxicology Vol. 30; no. 1; pp. 1 - 8
• Main Authors: Badkoubi, A. (Tarbiat Modarres University, Tehran.), Stevens, D.K, Muraraka, I.P
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer-Verlag 01.01.1996; Berlin; New York, NY
• Subjects: Biodegradation of pollutants; Biological and medical sciences; Biotechnology; COMPOSE ORGANOCHLORE; COMPOSE VOLATIL; COMPUESTO ORGANICO DEL CLORO; COMPUESTO VOLATIL; CULTURE TECHNIQUES; Environment and pollution; Fundamental and applied biological sciences. Psychology; Industrial applications and implications. Economical aspects; METABOLITE; METABOLITES; METABOLITOS; MINERALISATION; MINERALIZACION; MINERALIZATION; ORGANOCHLORINE COMPOUNDS; PHANEROCHAETE CHRYSOSPORIUM; SORCION; SORPTION; SPATIAL DISTRIBUTION; TECHNIQUE DE CULTURE; TECNICAS DE CULTIVO; VOLATILE COMPOUNDS; Volatile compound; Pesticides; Basidiomycetes; Fungi; Assay; Pollutant; Gas chromatography; Decontamination; Mineralization; Biotransformation; Phanerochaete chrysosporium; Phenols; Pentachlorophenol; Biocide; Degradation product; Thallophyta
• ISSN: 0090-4341; 1432-0703
• DOI: 10.1007/BF00211322

Experiments were carried out to quantify the mineralization and distribution of pentachlorophenol (PCP) by the white rot fungus Phanerochaete chrysosporium, and distribution of its transformation products into water-soluble, solvent-soluble, sorbed, and volatile fractions in liquid cultures. Water-soluble and solvent-soluble products from super(14)C-PCP transformation were first measured under oxygen limited conditions. Results indicated that after 12 days, 15% of super(14)C was recovered in methylene chloride, and less than 1% was water soluble. Sorption to the fungal mat reached a maximum of 16% super(14)C after 9 days incubation and declined to 5% at day 12. However, recovery of super(14)C within the system was only 30% at day 12. To improve the mass balance of the system, polyurethane foam was placed inside the culture flask to trap volatile products of PCP transformation. Results showed that after 12 days incubation of super(14)C-PCP with the fungus, 82% of the super(14)C added was volatilized. GC/MS analysis demonstrated that pentachloroanisole (PCA) was the only volatile product of PCP transformation. Sorption of super(14)C to the fungal mat was reversible to some extent. Chemical mass balance results in this experiment were near 100%. In the final experiment, the polyurethane volatile trap was placed outside the culture flask to assess the impact of the continuous removal of volatiles from the flask head space on mineralization. Increased super(14)CO sub(2) production was observed when the polyurethane volatile trap was placed outside the culture flask compared with placement inside the flask.