Cellulase in the host-parasite system Phaseolus vulgaris (L.): Uromyces appendiculatus [Pers.] Link

• Published in: European journal of plant pathology Vol. 105; no. 3; pp. 285 - 295
• Main Authors: Schmidt, C.S. (Aberdeen Univ. (United Kingdom). Dept. of Plant and Soil Science), Wolf, G.A
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer 01.04.1999; Springer Nature B.V
• Subjects: Beans; Biological and medical sciences; CELLULASE; CELULASA; ELECTROFORESIS; ELECTROPHORESE; ELECTROPHORESIS; Enzymes; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Immunization; Pathology, epidemiology, host-fungus relationships. Damages, economic importance; PHASEOLUS VULGARIS; Phytopathology. Animal pests. Plant and forest protection; UROMYCES APPENDICULATUS; Plant pathogen; Mycosis; Enzyme; Uromyces appendiculatus; Basidiomycetes; Host agent relation; Fungi; Infection; Grain legume; Leguminosae; Enzymatic activity; Cellulase; Phaseolus vulgaris; Dicotyledones; Glycosidases; Angiospermae; Hydrolases; Spermatophyta; Xylan endo-1,3-β-xylosidase; O-Glycosidases; Thallophyta
• ISSN: 0929-1873; 1573-8469
• DOI: 10.1023/A:1008763929057

Activity of cellulase and xylanase in the intercellular washing fluid (IWF) of bean plants (Phaseolus vulgaris, cultivar Fori) was monitored during infection with bean rust (Uromyces appendiculatus). In infected plants, cellulase activity could be detected at 2 days after inoculation and reached its maximum between 7 and 8 days after inoculation. The enzyme activity was not detected in healthy controls. The cellulase had a pH optimum at pH 5.5 and a temperature optimum at 30°C. Complete inactivation of cellulase occurred after heating to 50°C for 30min. In non-denaturing polyacrylamide gradient gels, the enzyme exhibited four bands (molecular masses approximately 70, 95, 120, 170kDa). After isoelectric focusing, eight cellulase isoforms with pI values pI 4.6-4.8; 5-5.1; 5.4; 5.5; 5.9; 6; 6.5; 7 appeared. Two dimensional electrophoresis yielded 13 cellulase isoforms. Unlike cellulase, low levels of xylanase were detected in healthy controls. The activity of this hydrolase did not increase due to rust infection.[PUBLICATION ABSTRACT]