Mechanism of Ca2+-mediated NOX modulated in ROS metabolism induced by T-2 toxin in potato tuber

•EGTA significantly influenced StNOXB in T-2 toxin-treated slices.•EGTA significantly influenced NOX activity on plant membrane and production of O2− and H2O2.•POD, CAT and SOD and ascorbate-glutathione cycle were markedly affected by Ca2+.•Ca2+ affects ROS metabolism and the activity of the antioxi...

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Published inFood chemistry Vol. 317; p. 126416
Main Authors Rui, Zhang, Huali, Xue, Min, Si, Yang, Bi, Mina, Nan, Yuanyuan, Zong, Haitao, Long, Prusky, Dov, Xiaoyan, Cheng
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.07.2020
Subjects
Ca2
calcium
cell membranes
chelating agents
EF hand motifs
EGTA
enzyme activity
ethylene glycol tetraacetic acid
gene expression
metabolism
NADPH oxidase
Potato tuber
potatoes
Reactive oxygen species
T-2 toxin
tubers
Reactive oxygen species
Ca2
NADPH oxidase
Potato tuber
EGTA
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Summary:•EGTA significantly influenced StNOXB in T-2 toxin-treated slices.•EGTA significantly influenced NOX activity on plant membrane and production of O2− and H2O2.•POD, CAT and SOD and ascorbate-glutathione cycle were markedly affected by Ca2+.•Ca2+ affects ROS metabolism and the activity of the antioxidant systems in tuber slices. T-2 toxin at low concentrations can induce ROS accumulation and modulate host resistance in plants. NOX plays crucial roles in ROS production and is regulated by Ca2+via direct binding to EF-hand motifs. In this study, the effect of EGTA (Ca2+ chelating agent) on the expression and enzymatic activity of NOX, as well as the activities and corresponding gene expressions involved in ROS metabolism and cell membrane integrity, were investigated in treated slices. Results indicated that EGTA treatment significantly affected gene expression and activity of NOX, and reduced ROS accumulation and cell membrane integrity and the enzymatic activities and gene expression involved in ROS metabolism when exposed to treatment. The addition of exogenous Ca2+ restored the initial relative transcript abundance, ROS accumulation and their activities. Results suggest that Ca2+ affected by EGTA plays a crucial role in NOX activity regulation, ultimately affecting ROS metabolism in slices induced by T-2 toxin.
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ISSN:0308-8146
1873-7072
1873-7072
DOI:10.1016/j.foodchem.2020.126416