Constriction model of actomyosin ring for cytokinesis by fission yeast using a two-state sliding filament mechanism

• Published in: The Journal of chemical physics Vol. 141; no. 12; p. 125101
• Main Authors: Jung, Yong-Woon, Mascagni, Michael
• Format: Journal Article
• Language: English
• Published: United States 28.09.2014
• Subjects: Actinin - metabolism; Actins - metabolism; Actomyosin - metabolism; Algorithms; Computer Simulation; Cytokinesis - physiology; Elasticity; Fungal Proteins - metabolism; Kinetics; Models, Biological; Myosins - metabolism; Sarcomeres - physiology; Schizosaccharomyces - physiology
• ISSN: 1089-7690
• DOI: 10.1063/1.4896164

We developed a model describing the structure and contractile mechanism of the actomyosin ring in fission yeast, Schizosaccharomyces pombe. The proposed ring includes actin, myosin, and α-actinin, and is organized into a structure similar to that of muscle sarcomeres. This structure justifies the use of the sliding-filament mechanism developed by Huxley and Hill, but it is probably less organized relative to that of muscle sarcomeres. Ring contraction tension was generated via the same fundamental mechanism used to generate muscle tension, but some physicochemical parameters were adjusted to be consistent with the proposed ring structure. Simulations allowed an estimate of ring constriction tension that reproduced the observed ring constriction velocity using a physiologically possible, self-consistent set of parameters. Proposed molecular-level properties responsible for the thousand-fold slower constriction velocity of the ring relative to that of muscle sarcomeres include fewer myosin molecules involved, a less organized contractile configuration, a low α-actinin concentration, and a high resistance membrane tension. Ring constriction velocity is demonstrated as an exponential function of time despite a near linear appearance. We proposed a hypothesis to explain why excess myosin heads inhibit constriction velocity rather than enhance it. The model revealed how myosin concentration and elastic resistance tension are balanced during cytokinesis in S. pombe.