The Comparison of RhoC and PI3K Gene Expression on the Breast Cancer Tissue and Benign Tumour Tissue
The expression of a gene is a process that conveys information of genes to synthesise gene product is functional. Alterations of the molecular biology in breast cancer are very complex because of many factors play a role in the tumorigenesis. is a prometastases gene. The gene is crucial in the regul...
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Published in | Open access Macedonian journal of medical sciences Vol. 7; no. 12; pp. 1911 - 1916 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
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Macedonia
Republic of Macedonia
30.06.2019
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Abstract | The expression of a gene is a process that conveys information of genes to synthesise gene product is functional. Alterations of the molecular biology in breast cancer are very complex because of many factors play a role in the tumorigenesis.
is a prometastases gene. The
gene is crucial in the regulation of multiple cellular functions, including cell growth, proliferation, metabolism and angiogenesis.
This study aims to compare of
and
gene expression on the breast cancer tissue and benign tumour tissue.
Expression of the
and
genes was carried out with qPCR. The absolute quantification method was using breast cancer tissue. As a comparison, benign tumours (FATs) tissue was carried out. The standard curves were obtained from cloning target genes, which were inserted into the gGEMT-easy vector from
. The gene expression data was carried out by t-test to see the mean difference between the expression of breast cancer tissue and benign tumours (FATs) with a value of p ≤ 0.005 in
and
gene expression. And the relationship between expressions was done by Pearson correlation test.
The results showed that it was found that
gene expression on breast cancer tissue was higher than the number in a benign tumour (fibroadenoma mammae) as an endogenous control. And also, the expression of
is much lower on breast cancer tissue compared with a benign tumour.
This study concluded that expression of
affects the expression of
so that the thing this is what causes the proliferation and began to provide support aggressive cancer cells in the breast. |
---|---|
AbstractList | BACKGROUND: The expression of a gene is a process that conveys information of genes to synthesise gene product is functional. Alterations of the molecular biology in breast cancer are very complex because of many factors play a role in the tumorigenesis. RhoC is a prometastases gene. The PI3K gene is crucial in the regulation of multiple cellular functions, including cell growth, proliferation, metabolism and angiogenesis.AIM: This study aims to compare of RhoC and PI3K gene expression on the breast cancer tissue and benign tumour tissue.MATERIAL AND METHODS: Expression of the RhoC and PI3K genes was carried out with qPCR. The absolute quantification method was using breast cancer tissue. As a comparison, benign tumours (FATs) tissue was carried out. The standard curves were obtained from cloning target genes, which were inserted into the gGEMT-easy vector from E. coli. The gene expression data was carried out by t-test to see the mean difference between the expression of breast cancer tissue and benign tumours (FATs) with a value of p ≤ 0.005 in RhoC and PI3K gene expression. And the relationship between expressions was done by Pearson correlation test.RESULTS: The results showed that it was found that PI3K gene expression on breast cancer tissue was higher than the number in a benign tumour (fibroadenoma mammae) as an endogenous control. And also, the expression of RhoC is much lower on breast cancer tissue compared with a benign tumour. CONCLUSION: This study concluded that expression of RhoC affects the expression of PI3K so that the thing this is what causes the proliferation and began to provide support aggressive cancer cells in the breast. The expression of a gene is a process that conveys information of genes to synthesise gene product is functional. Alterations of the molecular biology in breast cancer are very complex because of many factors play a role in the tumorigenesis. is a prometastases gene. The gene is crucial in the regulation of multiple cellular functions, including cell growth, proliferation, metabolism and angiogenesis. This study aims to compare of and gene expression on the breast cancer tissue and benign tumour tissue. Expression of the and genes was carried out with qPCR. The absolute quantification method was using breast cancer tissue. As a comparison, benign tumours (FATs) tissue was carried out. The standard curves were obtained from cloning target genes, which were inserted into the gGEMT-easy vector from . The gene expression data was carried out by t-test to see the mean difference between the expression of breast cancer tissue and benign tumours (FATs) with a value of p ≤ 0.005 in and gene expression. And the relationship between expressions was done by Pearson correlation test. The results showed that it was found that gene expression on breast cancer tissue was higher than the number in a benign tumour (fibroadenoma mammae) as an endogenous control. And also, the expression of is much lower on breast cancer tissue compared with a benign tumour. This study concluded that expression of affects the expression of so that the thing this is what causes the proliferation and began to provide support aggressive cancer cells in the breast. BACKGROUNDThe expression of a gene is a process that conveys information of genes to synthesise gene product is functional. Alterations of the molecular biology in breast cancer are very complex because of many factors play a role in the tumorigenesis. RhoC is a prometastases gene. The PI3K gene is crucial in the regulation of multiple cellular functions, including cell growth, proliferation, metabolism and angiogenesis. AIMThis study aims to compare of RhoC and PI3K gene expression on the breast cancer tissue and benign tumour tissue. MATERIAL AND METHODSExpression of the RhoC and PI3K genes was carried out with qPCR. The absolute quantification method was using breast cancer tissue. As a comparison, benign tumours (FATs) tissue was carried out. The standard curves were obtained from cloning target genes, which were inserted into the gGEMT-easy vector from E. coli. The gene expression data was carried out by t-test to see the mean difference between the expression of breast cancer tissue and benign tumours (FATs) with a value of p ≤ 0.005 in RhoC and PI3K gene expression. And the relationship between expressions was done by Pearson correlation test. RESULTSThe results showed that it was found that PI3K gene expression on breast cancer tissue was higher than the number in a benign tumour (fibroadenoma mammae) as an endogenous control. And also, the expression of RhoC is much lower on breast cancer tissue compared with a benign tumour. CONCLUSIONThis study concluded that expression of RhoC affects the expression of PI3K so that the thing this is what causes the proliferation and began to provide support aggressive cancer cells in the breast. |
Author | Arisanty, Dessy Harahap, Wirsma Arif Khambri, Daan Menra, Juane Plantika Rustam, Rony Aliska, Gestina Achyar, Affifatul |
AuthorAffiliation | 4 Biomedical Laboratory, Molecular Division, Faculty of Medicine, Andalas University, Padang, Indonesia 1 Department of Biochemistry, Faculty of Medicine, Andalas University, Padang, Indonesia 2 Division of Surgical Oncology, Medical School of Dr. M. Djamil Hospital, Andalas University, Padang, Indonesia 3 Department Farmacology, Faculty of Medicine, Andalas University, Padang, Indonesia |
AuthorAffiliation_xml | – name: 4 Biomedical Laboratory, Molecular Division, Faculty of Medicine, Andalas University, Padang, Indonesia – name: 1 Department of Biochemistry, Faculty of Medicine, Andalas University, Padang, Indonesia – name: 2 Division of Surgical Oncology, Medical School of Dr. M. Djamil Hospital, Andalas University, Padang, Indonesia – name: 3 Department Farmacology, Faculty of Medicine, Andalas University, Padang, Indonesia |
Author_xml | – sequence: 1 givenname: Dessy surname: Arisanty fullname: Arisanty, Dessy organization: Department of Biochemistry, Faculty of Medicine, Andalas University, Padang, Indonesia – sequence: 2 givenname: Wirsma Arif surname: Harahap fullname: Harahap, Wirsma Arif organization: Division of Surgical Oncology, Medical School of Dr. M. Djamil Hospital, Andalas University, Padang, Indonesia – sequence: 3 givenname: Daan surname: Khambri fullname: Khambri, Daan organization: Division of Surgical Oncology, Medical School of Dr. M. Djamil Hospital, Andalas University, Padang, Indonesia – sequence: 4 givenname: Rony surname: Rustam fullname: Rustam, Rony organization: Division of Surgical Oncology, Medical School of Dr. M. Djamil Hospital, Andalas University, Padang, Indonesia – sequence: 5 givenname: Gestina surname: Aliska fullname: Aliska, Gestina organization: Department Farmacology, Faculty of Medicine, Andalas University, Padang, Indonesia – sequence: 6 givenname: Affifatul surname: Achyar fullname: Achyar, Affifatul organization: Biomedical Laboratory, Molecular Division, Faculty of Medicine, Andalas University, Padang, Indonesia – sequence: 7 givenname: Juane Plantika surname: Menra fullname: Menra, Juane Plantika organization: Biomedical Laboratory, Molecular Division, Faculty of Medicine, Andalas University, Padang, Indonesia |
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Copyright | Copyright: © 2019 Dessy Arisanty, Wirsma Arif Harahap, Daan Khambri, Rony Rustam, Gestina Aliska, Affifatul Achyar, Juane Plantika Menra. 2019 |
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Keywords | PI3K gene Real-time PCR (qPCR) RhoC gene Vector Cloning |
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Title | The Comparison of RhoC and PI3K Gene Expression on the Breast Cancer Tissue and Benign Tumour Tissue |
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