The Comparison of RhoC and PI3K Gene Expression on the Breast Cancer Tissue and Benign Tumour Tissue
The expression of a gene is a process that conveys information of genes to synthesise gene product is functional. Alterations of the molecular biology in breast cancer are very complex because of many factors play a role in the tumorigenesis. is a prometastases gene. The gene is crucial in the regul...
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Published in | Open access Macedonian journal of medical sciences Vol. 7; no. 12; pp. 1911 - 1916 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Macedonia
Republic of Macedonia
30.06.2019
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Subjects | |
Online Access | Get full text |
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Summary: | The expression of a gene is a process that conveys information of genes to synthesise gene product is functional. Alterations of the molecular biology in breast cancer are very complex because of many factors play a role in the tumorigenesis.
is a prometastases gene. The
gene is crucial in the regulation of multiple cellular functions, including cell growth, proliferation, metabolism and angiogenesis.
This study aims to compare of
and
gene expression on the breast cancer tissue and benign tumour tissue.
Expression of the
and
genes was carried out with qPCR. The absolute quantification method was using breast cancer tissue. As a comparison, benign tumours (FATs) tissue was carried out. The standard curves were obtained from cloning target genes, which were inserted into the gGEMT-easy vector from
. The gene expression data was carried out by t-test to see the mean difference between the expression of breast cancer tissue and benign tumours (FATs) with a value of p ≤ 0.005 in
and
gene expression. And the relationship between expressions was done by Pearson correlation test.
The results showed that it was found that
gene expression on breast cancer tissue was higher than the number in a benign tumour (fibroadenoma mammae) as an endogenous control. And also, the expression of
is much lower on breast cancer tissue compared with a benign tumour.
This study concluded that expression of
affects the expression of
so that the thing this is what causes the proliferation and began to provide support aggressive cancer cells in the breast. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1857-9655 1857-9655 |
DOI: | 10.3889/oamjms.2019.543 |