In-well cell lysis technique reveals two new megaplasmids of 103.0 and 212.6 MDa in the multiple plasmid-containing strain V517 of Escherichia coli

• Published in: Letters in applied microbiology Vol. 34; no. 2; pp. 130 - 133
• Main Authors: Pedraza, R O, Díaz Ricci, J C
• Format: Journal Article
• Language: English
• Published: England 2002
• Subjects: Bacteriological Techniques; Bacteriolysis; Escherichia coli; Escherichia coli - genetics; Escherichia coli - physiology; Plasmids - genetics; Plasmids - isolation & purification; Plasmids - physiology

Identification of two new plasmids in the multiple plasmid-containing strain V517 of Escherichia coli. By using an in-well mild cell lysis technique suitable for megaplasmids observation, two plasmids of 103.0 and 212.6 MDa were detected in the multiplasmid-containing E. coli V517. The two new megap...