In-well cell lysis technique reveals two new megaplasmids of 103.0 and 212.6 MDa in the multiple plasmid-containing strain V517 of Escherichia coli

Identification of two new plasmids in the multiple plasmid-containing strain V517 of Escherichia coli. By using an in-well mild cell lysis technique suitable for megaplasmids observation, two plasmids of 103.0 and 212.6 MDa were detected in the multiplasmid-containing E. coli V517. The two new megap...

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Published inLetters in applied microbiology Vol. 34; no. 2; pp. 130 - 133
Main Authors Pedraza, R O, Díaz Ricci, J C
Format Journal Article
LanguageEnglish
Published England 2002
Subjects
Bacteriological Techniques
Bacteriolysis
Escherichia coli
Escherichia coli - genetics
Escherichia coli - physiology
Plasmids - genetics
Plasmids - isolation & purification
Plasmids - physiology
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Summary:Identification of two new plasmids in the multiple plasmid-containing strain V517 of Escherichia coli. By using an in-well mild cell lysis technique suitable for megaplasmids observation, two plasmids of 103.0 and 212.6 MDa were detected in the multiplasmid-containing E. coli V517. The two new megaplasmids that were completely overlooked when standard disruptive procedures were used, can now be added to the list of eight plasmids with molecular size from 1.36 to 35.84 MDa reported earlier. This finding allows to use the strain V517 not only as a size reference of small and moderately large plasmids but as a size reference of megaplasmids as well.
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ISSN:0266-8254
1365-2673
DOI:10.1046/j.1472-765x.2002.01059.x