The use of hemolysis kinetics to evaluate erythrocyte-bound surfactant

The partition of surfactant between erythrocytes and their suspending medium was evaluated using the mass-conservation law and a partition model for the surfactant. Contrary to previous work, it is demonstrated that such an evaluation has to be done using results for the whole time range of hemolysi...

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Bibliographic Details
Published inColloids and surfaces, B, Biointerfaces Vol. 27; no. 2; pp. 215 - 222
Main Authors Shalel, Sagit, Streichman, Sara, Marmur, Abraham
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.02.2003
Subjects
Erythrocyte
Hemolysis
Membrane-bound surfactant
Partition coefficient
Surfactant
Hemolysis
Erythrocyte
Partition coefficient
Surfactant
Membrane-bound surfactant
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Summary:The partition of surfactant between erythrocytes and their suspending medium was evaluated using the mass-conservation law and a partition model for the surfactant. Contrary to previous work, it is demonstrated that such an evaluation has to be done using results for the whole time range of hemolysis. The use of a single time-point, for which a certain amount of hemolysis is achieved, may, in some cases, result in misleading conclusions. The present analysis implies that equilibrium is maintained between the membrane-bound surfactant and the bulk surfactant throughout the hemolysis process, in spite of the release of cell interior. This equilibrium may be described by a simple partition model, which is characterized by a constant partition coefficient. A practical approach to the evaluation of the surfactant partition, which is particularly useful when the whole hemolysis curve needs to be studied, is suggested.
ISSN:0927-7765
1873-4367
DOI:10.1016/S0927-7765(02)00074-7