Introduction of O-butyrylchitosan with a photosensitive hetero-bifunctional crosslinking reagent to silicone rubber film by radiation grafting and its blood compatibility

• Published in: Colloids and surfaces, B, Biointerfaces Vol. 30; no. 4; pp. 299 - 306
• Main Authors: Mao, C., Zhu, A.P., Qiu, Y.Z., Shen, J., Lin, S.C.
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.08.2003
• Subjects: Blood compatibility; O-butyrylchitosan; Platelet adhesion; Radiation grafting; Silicone rubber; Surface modification; Radiation grafting; Surface modification; Blood compatibility; Platelet adhesion; O-butyrylchitosan; Silicone rubber
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/S0927-7765(03)00115-2

Based on an in vitro test for an improvement of the blood compatibility of silicone rubber (SR) films by grafting O-butyrylchitosan (OBCS), OBCS was covalently immobilized onto SR film surface using the photosensitive hetero-bifunctional crosslinking reagent, 4-azidobenzoic acid, which was previously bonded to OBCS by reaction between an acid group of the crosslinking reagent and a free amino group of OBCS. Surface properties of SR film were investigated by attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), electron spectroscopy for chemical analysis (ESCA) and the water contact angle measurements. The blood compatibility of SR film was evaluated by platelet rich plasma (PRP) contacting experiments and the results were observed by scanning electron microscopy (SEM). The state of platelet adhesion was described. The suitable modifications could be carried out to tailor SR films biomaterial to meet the specific needs of different biomedical applications. These results suggest that the blood compatible of SR films/OBCS films show their suitability as potential biomaterials.